1zjb

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{{STRUCTURE_1zjb| PDB=1zjb | SCENE= }}
{{STRUCTURE_1zjb| PDB=1zjb | SCENE= }}
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'''Crystal structure of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45 (monoclinic form)'''
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===Crystal structure of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45 (monoclinic form)===
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==Overview==
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The trehalulose synthase (MutB) from Pseudomonas mesoacidophila MX-45, belonging to glycoside hydrolase family 13, catalyses the isomerization of sucrose to trehalulose (alpha-D-glucosylpyranosyl-1,1-D-fructofuranose) and isomaltulose (alpha-D-glucosylpyranosyl-1,6-D-fructofuranose) as main products and glucose and fructose in residual amounts from the hydrolytic reaction. To date, a three-dimensional structure of a sucrose isomerase that produces mainly trehalulose, as is the case for MutB, has been lacking. Crystallographic studies of this 64 kDa enzyme have therefore been initiated in order to contribute to the understanding of the molecular basis of sucrose decomposition, isomerization and of the selectivity of this enzyme that leads to the formation of different products. The MutB protein has been overexpressed, purified and crystallized using the hanging-drop vapour-diffusion method. Two different crystal forms have been obtained: one diffracts X-rays to 1.6 A resolution using synchrotron radiation and belongs to space group P1, with unit-cell parameters a = 63.8, b = 72.0, c = 82.2 A, alpha = 67.5, beta = 73.1, gamma = 70.8 degrees, while the other form diffracts to 1.8 A resolution using synchrotron radiation and belongs to space group P2(1), with unit-cell parameters a = 63.7, b = 85.9, c = 119.7 A, beta = 97.7 degrees. A molecular-replacement solution has been found using the structure of the isomaltulose synthase (PalI) from Klebsiella sp. LX3 as a search model.
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{{ABSTRACT_PUBMED_16508103}}
==About this Structure==
==About this Structure==
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[[Category: Sucrose isomerase]]
[[Category: Sucrose isomerase]]
[[Category: Trehalulose synthase]]
[[Category: Trehalulose synthase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:08:33 2008''

Revision as of 21:08, 27 July 2008

Image:1zjb.png

Template:STRUCTURE 1zjb

Crystal structure of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45 (monoclinic form)

Template:ABSTRACT PUBMED 16508103

About this Structure

1ZJB is a Single protein structure of sequence from Pseudomonas mesoacidophila. Full crystallographic information is available from OCA.

Reference

Expression, purification, crystallization and preliminary X-ray crystallographic studies of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45., Ravaud S, Watzlawick H, Haser R, Mattes R, Aghajari N, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):100-3. Epub 2004 Dec 2. PMID:16508103

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