3tmn
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(New page: 200px<br /><applet load="3tmn" size="450" color="white" frame="true" align="right" spinBox="true" caption="3tmn, resolution 1.7Å" /> '''THE BINDING OF L-VALY...)
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Revision as of 17:52, 20 November 2007
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THE BINDING OF L-VALYL-L-TRYPTOPHAN TO CRYSTALLINE THERMOLYSIN ILLUSTRATES THE MODE OF INTERACTION OF A PRODUCT OF PEPTIDE HYDROLYSIS
Overview
Crystallographic analysis of the binding of, mercaptoacetyl-L-valyl-L-tryptophan to thermolysin suggests that this, inhibitor is hydrolyzed by the crystalline enzyme. The apparent product of, hydrolysis, L-valyl-L-tryptophan (Val-Trp), occupies the S1'-S2' subsites, of the active site, not the S1-S1' subsites as observed previously for the, dipeptide L-alanyl-L-phenylalanine (Ala-Phe). The difference in binding of, Val-Trp and Ala-Phe is consistent with the specificity requirements and, preferences of thermolysin. The binding of Val-Trp illustrates the mode of, interaction of one of the products of peptide hydrolysis. High resolution, crystallographic refinement indicates that the valyl amino group makes, three hydrogen bonds to the enzyme and to solvent and, in addition, is 2.8, A from the carboxylate of Glu-143. This is the first instance in which a, direct interaction has been observed between Glu-143 and the scissile, nitrogen. As such, the study directly supports the mechanism of action for, thermolysin proposed by Hangauer et al. (Hangauer, D. G., Monzingo, A. F., and Matthews, B. W. (1984) Biochemistry 23, 5730-5741) and, by analogy, indirectly supports the similar mechanism proposed for carboxypeptidase A, (Monzingo, A. F., and Matthews, B. W. (1984) Biochemistry 23, 5724-5729).
About this Structure
3TMN is a Single protein structure of sequence from Bacillus thermoproteolyticus with CA and ZN as ligands. Active as Thermolysin, with EC number 3.4.24.27 Full crystallographic information is available from OCA.
Reference
The binding of L-valyl-L-tryptophan to crystalline thermolysin illustrates the mode of interaction of a product of peptide hydrolysis., Holden HM, Matthews BW, J Biol Chem. 1988 Mar 5;263(7):3256-60. PMID:3343246
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