This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2hfh

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2hfh.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2hfh.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2hfh| PDB=2hfh | SCENE= }}
{{STRUCTURE_2hfh| PDB=2hfh | SCENE= }}
-
'''THE NMR STRUCTURES OF A WINGED HELIX PROTEIN: GENESIS, 20 STRUCTURES'''
+
===THE NMR STRUCTURES OF A WINGED HELIX PROTEIN: GENESIS, 20 STRUCTURES===
-
==Overview==
+
<!--
-
The hepatocyte nuclear factor 3 (HNF-3)/fork head (fkh) family contains a large number of transcription factors and folds into a winged helix motif. Despite having almost invariable amino acid sequences in their principal DNA-binding helices, HNF-3/fkh proteins show a wide diversity of sequence-specific binding. Previous studies of chimeric HNF-3/fkh proteins demonstrated that the binding specificity is primarily influenced by a region directly adjacent to the binding helix. We report our findings of an NMR structural study performed on an HNF-3/fkh family member (Genesis, formerly HFH-2) and compare it to that of another family member (HNF-3gamma) complexed to DNA and determined by X-ray crystallography. It is found that in comparison to HNF-3gamma, Genesis contains an extra small helix directly prior to the N terminus of the primary DNA contact helix. Due to the insertion of this helix, a shorter and slightly re-positioned primary DNA contact helix is observed, which we believe leads to the DNA-binding specificity differences among family members.
+
The line below this paragraph, {{ABSTRACT_PUBMED_9571051}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 9571051 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_9571051}}
==About this Structure==
==About this Structure==
-
2HFH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HFH OCA].
+
2HFH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HFH OCA].
==Reference==
==Reference==
Line 27: Line 31:
[[Category: Hnf-3 homologue]]
[[Category: Hnf-3 homologue]]
[[Category: Winged helix protein]]
[[Category: Winged helix protein]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:13:47 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:13:16 2008''

Revision as of 21:13, 27 July 2008

Image:2hfh.png

Template:STRUCTURE 2hfh

THE NMR STRUCTURES OF A WINGED HELIX PROTEIN: GENESIS, 20 STRUCTURES

Template:ABSTRACT PUBMED 9571051

About this Structure

2HFH is a Single protein structure of sequence from Rattus norvegicus. Full experimental information is available from OCA.

Reference

Structural changes in the region directly adjacent to the DNA-binding helix highlight a possible mechanism to explain the observed changes in the sequence-specific binding of winged helix proteins., Marsden I, Jin C, Liao X, J Mol Biol. 1998 May 1;278(2):293-9. PMID:9571051

Page seeded by OCA on Mon Jul 28 00:13:16 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2hfh"
Personal tools