1r76

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{{STRUCTURE_1r76| PDB=1r76 | SCENE= }}
{{STRUCTURE_1r76| PDB=1r76 | SCENE= }}
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'''Structure of a pectate lyase from Azospirillum irakense'''
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===Structure of a pectate lyase from Azospirillum irakense===
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==Overview==
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The three-dimensional structure of Azospirillum irakense pectate lyase (PelA) has been determined at a resolution of 2.65 A. The crystals are hexagonal, belonging to space group P6(5)22, with unit-cell parameters a = b = 85.37, c = 231.32 angstroms. Phase information was derived from a multiple-wavelength anomalous dispersion (MAD) experiment using a Hg derivative. Refinement of the model converged to Rcryst = 20.08% and Rfree = 25.87%. The overall structure of PelA does not adopt the characteristic parallel beta-helix fold displayed by pectate lyases from polysaccharide lyase (PL) families PL1, PL3 and PL9. Instead, it displays a predominantly alpha-helical structure with irregular coils and short beta-strands, similar to the recently reported structure of the catalytic module of the Cellvibrio japonicus pectate lyase Pel10Acm. The topologies of the two structures have been compared. They show two 'domains' with the interface between them being a wide-open central groove in which the active site is located. The active sites of the crystal structures are also compared and their similarities and differences are discussed.
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(as it appears on PubMed at http://www.pubmed.gov), where 15159558 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15159558}}
==About this Structure==
==About this Structure==
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[[Category: Verboven, C.]]
[[Category: Verboven, C.]]
[[Category: A-helical structure]]
[[Category: A-helical structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:10:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:14:34 2008''

Revision as of 21:14, 27 July 2008

Image:1r76.png

Template:STRUCTURE 1r76

Structure of a pectate lyase from Azospirillum irakense

Template:ABSTRACT PUBMED 15159558

About this Structure

1R76 is a Single protein structure of sequence from Azospirillum irakense. Full crystallographic information is available from OCA.

Reference

Azospirillum irakense pectate lyase displays a toroidal fold., Novoa De Armas H, Verboven C, De Ranter C, Desair J, Vande Broek A, Vanderleyden J, Rabijns A, Acta Crystallogr D Biol Crystallogr. 2004 Jun;60(Pt 6):999-1007. Epub 2004, May 21. PMID:15159558

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