This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1svy

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1svy.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1svy.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1svy| PDB=1svy | SCENE= }}
{{STRUCTURE_1svy| PDB=1svy | SCENE= }}
-
'''SEVERIN DOMAIN 2, 1.75 ANGSTROM CRYSTAL STRUCTURE'''
+
===SEVERIN DOMAIN 2, 1.75 ANGSTROM CRYSTAL STRUCTURE===
-
==Overview==
+
<!--
-
The crystal structure of the F-actin binding domain 2 of severin, the gelsolin homologue from Dictyostelium discoideum, has been determined by multiple isomorphous replacement and refined to 1.75 A resolution. The structure reveals an alpha-helix-beta-sheet sandwich similar to the domains of gelsolin and villin, and contains two cation-binding sites, as observed in other domain 1 and domain 2 homologues. Comparison of the structures of several gelsolin family domains has identified residues that may mediate F-actin binding in gelsolin domain 2 homologues. To assess the involvement of these residues in F-actin binding, three mutants of human gelsolin domain 2 were assayed for F-actin binding activity and thermodynamic stability. Two of the mutants, RRV168AAA and RLK210AAA, demonstrated a lowered affinity for F-actin, indicating a role for those residues in filament binding. Using both structural and biochemical data, we have constructed a model of the gelsolin domain 1-domain 2-F-actin complex. This model highlights a number of interactions that may serve as positive and negative determinants of filament end- and side-binding.
+
The line below this paragraph, {{ABSTRACT_PUBMED_10820002}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 10820002 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_10820002}}
==About this Structure==
==About this Structure==
Line 36: Line 40:
[[Category: Severin]]
[[Category: Severin]]
[[Category: Villin]]
[[Category: Villin]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:12:01 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:24:54 2008''

Revision as of 21:24, 27 July 2008

Image:1svy.png

Template:STRUCTURE 1svy

SEVERIN DOMAIN 2, 1.75 ANGSTROM CRYSTAL STRUCTURE

Template:ABSTRACT PUBMED 10820002

About this Structure

1SVY is a Single protein structure of sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA.

Reference

Mapping the functional surface of domain 2 in the gelsolin superfamily., Puius YA, Fedorov EV, Eichinger L, Schleicher M, Almo SC, Biochemistry. 2000 May 9;39(18):5322-31. PMID:10820002

Page seeded by OCA on Mon Jul 28 00:24:54 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1svy"
Personal tools