2fpu

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{{STRUCTURE_2fpu| PDB=2fpu | SCENE= }}
{{STRUCTURE_2fpu| PDB=2fpu | SCENE= }}
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'''Crystal Structure of the N-terminal domain of E.coli HisB- Complex with histidinol'''
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===Crystal Structure of the N-terminal domain of E.coli HisB- Complex with histidinol===
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==Overview==
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HisB from Escherichia coli is a bifunctional enzyme catalyzing the sixth and eighth steps of l-histidine biosynthesis. The N-terminal domain (HisB-N) possesses histidinol phosphate phosphatase activity, and its crystal structure shows a single domain with fold similarity to the haloacid dehalogenase (HAD) enzyme family. HisB-N forms dimers in the crystal and in solution. The structure shows the presence of a structural Zn(2+) ion stabilizing the conformation of an extended loop. Two metal binding sites were also identified in the active site. Their presence was further confirmed by isothermal titration calorimetry. HisB-N is active in the presence of Mg(2+), Mn(2+), Co(2+), or Zn(2+), but Ca(2+) has an inhibitory effect. We have determined structures of several intermediate states corresponding to snapshots along the reaction pathway, including that of the phosphoaspartate intermediate. A catalytic mechanism, different from that described for other HAD enzymes, is proposed requiring the presence of the second metal ion not found in the active sites of previously characterized HAD enzymes, to complete the second half-reaction. The proposed mechanism is reminiscent of two-Mg(2+) ion catalysis utilized by DNA and RNA polymerases and many nucleases. The structure also provides an explanation for the inhibitory effect of Ca(2+).
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{{ABSTRACT_PUBMED_16966333}}
==About this Structure==
==About this Structure==
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[[Category: Montreal-kingston bacterial structural genomics initiative]]
[[Category: Montreal-kingston bacterial structural genomics initiative]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:30:09 2008''

Revision as of 21:30, 27 July 2008

Image:2fpu.png

Template:STRUCTURE 2fpu

Crystal Structure of the N-terminal domain of E.coli HisB- Complex with histidinol

Template:ABSTRACT PUBMED 16966333

About this Structure

2FPU is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural snapshots of Escherichia coli histidinol phosphate phosphatase along the reaction pathway., Rangarajan ES, Proteau A, Wagner J, Hung MN, Matte A, Cygler M, J Biol Chem. 2006 Dec 8;281(49):37930-41. Epub 2006 Sep 11. PMID:16966333

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