2c51

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{{STRUCTURE_2c51| PDB=2c51 | SCENE= }}
{{STRUCTURE_2c51| PDB=2c51 | SCENE= }}
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'''MS2-RNA HAIRPIN (G-5) COMPLEX'''
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===MS2-RNA HAIRPIN (G-5) COMPLEX===
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==Overview==
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We have determined the X-ray structures of six MS2 RNA hairpin-coat-protein complexes having five different substitutions at the hairpin loop base -5. This is a uracil in the wild-type hairpin and contacts the coat protein both by stacking on to a tyrosine side chain and by hydrogen bonding to an asparagine side chain. The RNA consensus sequence derived from coat protein binding studies with natural sequence variants suggested that the -5 base needs to be a pyrimidine for strong binding. The five -5 substituents used in this study were 5-bromouracil, pyrimidin-2-one, 2-thiouracil, adenine, and guanine. The structure of the 5-bromouracil complex was determined to 2.2 A resolution, which is the highest to date for any MS2 RNA-protein complex. All the complexes presented here show very similar conformations, despite variation in affinity in solution. The results suggest that the stacking of the -5 base on to the tyrosine side chain is the most important driving force for complex formation. A number of hydrogen bonds that are present in the wild-type complex are not crucial for binding, as they are missing in one or more of the complexes. The results also reveal the flexibility of this RNA-protein interface, with respect to functional group variation, and may be generally applicable to other RNA-protein complexes.
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{{ABSTRACT_PUBMED_11720290}}
==About this Structure==
==About this Structure==
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[[Category: Virus coat protein]]
[[Category: Virus coat protein]]
[[Category: Virus/viral protein/rna]]
[[Category: Virus/viral protein/rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:42:08 2008''

Revision as of 21:42, 27 July 2008

Image:2c51.png

Template:STRUCTURE 2c51

MS2-RNA HAIRPIN (G-5) COMPLEX

Template:ABSTRACT PUBMED 11720290

About this Structure

2C51 is a Protein complex structure of sequences from Enterobacterio phage ms2. This structure supersedes the now removed PDB entry 1h8j. Full crystallographic information is available from OCA.

Reference

Structural basis of pyrimidine specificity in the MS2 RNA hairpin-coat-protein complex., Grahn E, Moss T, Helgstrand C, Fridborg K, Sundaram M, Tars K, Lago H, Stonehouse NJ, Davis DR, Stockley PG, Liljas L, RNA. 2001 Nov;7(11):1616-27. PMID:11720290

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