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1p35

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{{STRUCTURE_1p35| PDB=1p35 | SCENE= }}
{{STRUCTURE_1p35| PDB=1p35 | SCENE= }}
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'''CRYSTAL STRUCTURE OF BACULOVIRUS P35'''
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===CRYSTAL STRUCTURE OF BACULOVIRUS P35===
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==Overview==
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The aspartate-specific caspases are critical protease effectors of programmed cell death and consequently represent important targets for apoptotic intervention. Baculovirus P35 is a potent substrate inhibitor of metazoan caspases, a property that accounts for its unique effectiveness in preventing apoptosis in phylogenetically diverse organisms. Here we report the 2.2 A resolution crystal structure of P35, the first structure of a protein inhibitor of the death caspases. The P35 monomer possesses a solvent-exposed loop that projects from the protein's main beta-sheet core and positions the requisite aspartate cleavage site at the loop's apex. Distortion or destabilization of this reactive site loop by site-directed mutagenesis converted P35 to an efficient substrate which, unlike wild-type P35, failed to interact stably with the target caspase or block protease activity. Thus, cleavage alone is insufficient for caspase inhibition. These data are consistent with a new model wherein the P35 reactive site loop participates in a unique multi-step mechanism in which the spatial orientation of the loop with respect to the P35 core determines post-cleavage association and stoichiometric inhibition of target caspases.
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(as it appears on PubMed at http://www.pubmed.gov), where 10205157 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10205157}}
==About this Structure==
==About this Structure==
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[[Category: Cell death]]
[[Category: Cell death]]
[[Category: P35]]
[[Category: P35]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:37:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:44:58 2008''

Revision as of 21:45, 27 July 2008

Image:1p35.png

Template:STRUCTURE 1p35

CRYSTAL STRUCTURE OF BACULOVIRUS P35

Template:ABSTRACT PUBMED 10205157

About this Structure

1P35 is a Single protein structure of sequence from Autographa californica nucleopolyhedrovirus. Full crystallographic information is available from OCA.

Reference

Crystal structure of baculovirus P35: role of a novel reactive site loop in apoptotic caspase inhibition., Fisher AJ, Cruz W, Zoog SJ, Schneider CL, Friesen PD, EMBO J. 1999 Apr 15;18(8):2031-9. PMID:10205157

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