1kzn
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(New page: 200px<br /><applet load="1kzn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kzn, resolution 2.30Å" /> '''Crystal Structure of...)
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Revision as of 17:59, 20 November 2007
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Crystal Structure of E. coli 24kDa Domain in Complex with Clorobiocin
Overview
DNA gyrase is a major bacterial protein that is involved in replication, and transcription and catalyzes the negative supercoiling of bacterial, circular DNA. DNA gyrase is a known target for antibacterial agents since, its blocking induces bacterial death. Quinolones, coumarins, and, cyclothialidines have been designed to inhibit gyrase. Significant, improvements can still be envisioned for a better coumarin-gyrase, interaction. In this work, we obtained the crystal costructures of the, natural coumarin clorobiocin and a synthetic analogue with the 24 kDa, gyrase fragment. We used isothermal titration microcalorimetry and, differential scanning calorimetry to obtain the thermodynamic parameters, representative of the molecular interactions occurring during the binding, process between coumarins and the 24 kDa gyrase fragment. We provide the, first experimental evidence that clorobiocin binds gyrase with a stronger, affinity than novobiocin. We also demonstrate the crucial role of both the, hydroxybenzoate isopentenyl moiety and the 5'-alkyl group on the noviose, of the coumarins in the binding affinity for gyrase.
About this Structure
1KZN is a Single protein structure of sequence from Escherichia coli with CBN as ligand. Active as DNA topoisomerase (ATP-hydrolyzing), with EC number 5.99.1.3 Full crystallographic information is available from OCA.
Reference
DNA gyrase interaction with coumarin-based inhibitors: the role of the hydroxybenzoate isopentenyl moiety and the 5'-methyl group of the noviose., Lafitte D, Lamour V, Tsvetkov PO, Makarov AA, Klich M, Deprez P, Moras D, Briand C, Gilli R, Biochemistry. 2002 Jun 11;41(23):7217-23. PMID:12044152
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