2p6p

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2p6p.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2p6p.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2p6p| PDB=2p6p | SCENE= }}
{{STRUCTURE_2p6p| PDB=2p6p | SCENE= }}
-
'''X-ray crystal structure of C-C bond-forming dTDP-D-Olivose-transferase UrdGT2'''
+
===X-ray crystal structure of C-C bond-forming dTDP-D-Olivose-transferase UrdGT2===
-
==Overview==
+
<!--
-
The glycosyltransferase UrdGT2 from Streptomyces fradiae catalyzes the formation of a glycosidic C-C bond between a polyketide aglycone and D-olivose. The enyzme was expressed in Escherichia coli, purified and crystallized. Its structure was established by X-ray diffraction at 1.9 A resolution. It is the first structure of a C-glycosyltransferase. UrdGT2 belongs to the structural family GT-B of the glycosyltransferases and is likely to form a C(2)-symmetric dimer in solution. The binding structures of donor and acceptor substrates in five structurally homologous enzymes provided a clear and consistent guide for the substrate-binding structure in UrdGT2. The modeled substrate locations suggest the deeply buried Asp137 as the activator for C-C bond formation and explain the reaction. The putative model can be used to design mutations that change the substrate specificity. Such mutants are of great interest in overcoming the increasing danger of antibiotic resistance.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17640665}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17640665 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17640665}}
==About this Structure==
==About this Structure==
Line 26: Line 30:
[[Category: Gt-b family]]
[[Category: Gt-b family]]
[[Category: X-ray-diffraction,urdamycina-biosynthesis]]
[[Category: X-ray-diffraction,urdamycina-biosynthesis]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:29:45 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 01:06:38 2008''

Revision as of 22:06, 27 July 2008

Image:2p6p.png

Template:STRUCTURE 2p6p

X-ray crystal structure of C-C bond-forming dTDP-D-Olivose-transferase UrdGT2

Template:ABSTRACT PUBMED 17640665

About this Structure

Full crystallographic information is available from OCA.

Reference

Structure and action of the C-C bond-forming glycosyltransferase UrdGT2 involved in the biosynthesis of the antibiotic urdamycin., Mittler M, Bechthold A, Schulz GE, J Mol Biol. 2007 Sep 7;372(1):67-76. Epub 2007 Jun 9. PMID:17640665

Page seeded by OCA on Mon Jul 28 01:06:38 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2p6p"
Personal tools