1tqr

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[[Image:1tqr.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1tqr| PDB=1tqr | SCENE= }}
{{STRUCTURE_1tqr| PDB=1tqr | SCENE= }}
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'''NMR Structure of DNA 17-mer GGAAAATCTCTAGCAGT corresponding to the extremity of the U5 LTR of the HIV-1 genome'''
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===NMR Structure of DNA 17-mer GGAAAATCTCTAGCAGT corresponding to the extremity of the U5 LTR of the HIV-1 genome===
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==Overview==
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The integration of the human immunodeficiency virus type 1 DNA into the host cell genome is catalysed by the viral integrase (IN). The reaction consists of a 3'-processing [dinucleotide released from each 3' end of the viral long terminal repeat (LTR)] followed by a strand transfer (insertion of the viral genome into the human chromosome). A 17 base pair oligonucleotide d(GGAAAATCTCTAGCAGT), d(ACTGCTAGAGATTTTCC) reproducing the U5-LTR extremity of viral DNA that contains the IN attachment site was analysed by NMR using the classical NOEs and scalar coupling constants in conjunction with a small set of residual dipolar coupling constants (RDCs) measured at the 13C/15N natural abundance. The combination of these two types of parameters in calculations significantly improved the DNA structure determination. The well-known features of A-tracts were clearly identified by RDCs in the first part of the molecule. The binding/cleavage site at the viral DNA end is distinguishable by a loss of regular base stacking and a distorted minor groove that can aid its specific recognition by IN.
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The line below this paragraph, {{ABSTRACT_PUBMED_15814814}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15814814 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15814814}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TQR OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TQR OCA].
==Reference==
==Reference==
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[[Category: Renisio, J G.]]
[[Category: Renisio, J G.]]
[[Category: Double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:15:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 01:20:07 2008''

Revision as of 22:20, 27 July 2008

Image:1tqr.png

Template:STRUCTURE 1tqr

NMR Structure of DNA 17-mer GGAAAATCTCTAGCAGT corresponding to the extremity of the U5 LTR of the HIV-1 genome

Template:ABSTRACT PUBMED 15814814

About this Structure

Full experimental information is available from OCA.

Reference

Pre-organized structure of viral DNA at the binding-processing site of HIV-1 integrase., Renisio JG, Cosquer S, Cherrak I, El Antri S, Mauffret O, Fermandjian S, Nucleic Acids Res. 2005 Apr 6;33(6):1970-81. Print 2005. PMID:15814814

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