1n1d

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{{STRUCTURE_1n1d| PDB=1n1d | SCENE= }}
{{STRUCTURE_1n1d| PDB=1n1d | SCENE= }}
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'''Glycerol-3-phosphate cytidylyltransferase complexed with CDP-glycerol'''
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===Glycerol-3-phosphate cytidylyltransferase complexed with CDP-glycerol===
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==Overview==
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The bacterial enzyme, glycerol-3-phosphate cytidylyltransferase (GCT), is a model for mammalian cytidylyltransferases and is a member of a large superfamily of nucleotidyltransferases. Dimeric GCT from Bacillus subtilis displays unusual negative cooperativity in substrate binding and appears to form products only when both active sites are occupied by substrates. Here we describe a complex of GCT with the product, CDP-glycerol, in a crystal structure in which bound sulfate serves as a partial mimic of the second product, pyrophosphate. Binding of sulfate to form a pseudo-ternary complex is observed in three of the four chains constituting the asymmetric unit and is accompanied by a backbone rearrangement at Asp11 and ordering of the C-terminal helix. Comparison with the CTP complex of GCT, determined previously, reveals that in the product complex the active site closes around the glycerol phosphate moiety with a concerted motion of the segment 37-47 that includes helix B. This rearrangement allows lysines 44 and 46 to interact with the glycerol and cytosine phosphates of CDP-glycerol. Binding of CDP-glycerol also induces smaller movements of residues 92-100. Roles of lysines 44 and 46 in catalysis have been confirmed by mutagenesis of these residues to alanine, which decreases Vmax(app) and has profound effects on the Km(app) for glycerol-3-phosphate.
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{{ABSTRACT_PUBMED_14506262}}
==About this Structure==
==About this Structure==
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[[Category: Negative cooperativity]]
[[Category: Negative cooperativity]]
[[Category: Nucleotidyltransferase]]
[[Category: Nucleotidyltransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:58:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 01:30:48 2008''

Revision as of 22:31, 27 July 2008

Image:1n1d.png

Template:STRUCTURE 1n1d

Glycerol-3-phosphate cytidylyltransferase complexed with CDP-glycerol

Template:ABSTRACT PUBMED 14506262

About this Structure

1N1D is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Glycerol-3-phosphate cytidylyltransferase. Structural changes induced by binding of CDP-glycerol and the role of lysine residues in catalysis., Pattridge KA, Weber CH, Friesen JA, Sanker S, Kent C, Ludwig ML, J Biol Chem. 2003 Dec 19;278(51):51863-71. Epub 2003 Sep 23. PMID:14506262

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