1zfl

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{{STRUCTURE_1zfl| PDB=1zfl | SCENE= }}
{{STRUCTURE_1zfl| PDB=1zfl | SCENE= }}
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'''Solution structure of III-A, the major intermediate in the oxidative folding of leech carboxypeptidase inhibitor'''
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===Solution structure of III-A, the major intermediate in the oxidative folding of leech carboxypeptidase inhibitor===
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==Overview==
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The III-A intermediate constitutes the major rate-determining step in the oxidative folding of leech carboxypeptidase inhibitor (LCI). In this work, III-A has been directly purified from the folding reaction and structurally characterized by NMR spectroscopy. This species, containing three native disulfides, displays a highly native-like structure; however, it lacks some secondary structure elements, making it more flexible than native LCI. III-A represents a structurally determined example of a disulfide-insecure intermediate; direct oxidation of this species to the fully native protein seems to be restricted by the burial of its two free cysteine residues inside a native-like structure. We also show that theoretical approaches based on topological constraints predict with good accuracy the presence of this folding intermediate. Overall, the derived results suggest that, as it occurs with non-disulfide bonded proteins, native-like interactions between segments of secondary structure rather than the crosslinking of disulfide bonds direct the folding of LCI.
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The line below this paragraph, {{ABSTRACT_PUBMED_16084391}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16084391 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16084391}}
==About this Structure==
==About this Structure==
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1ZFL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZFL OCA].
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1ZFL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZFL OCA].
==Reference==
==Reference==
NMR structural characterization and computational predictions of the major intermediate in oxidative folding of leech carboxypeptidase inhibitor., Arolas JL, D'Silva L, Popowicz GM, Aviles FX, Holak TA, Ventura S, Structure. 2005 Aug;13(8):1193-202. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16084391 16084391]
NMR structural characterization and computational predictions of the major intermediate in oxidative folding of leech carboxypeptidase inhibitor., Arolas JL, D'Silva L, Popowicz GM, Aviles FX, Holak TA, Ventura S, Structure. 2005 Aug;13(8):1193-202. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16084391 16084391]
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Structure of a novel leech carboxypeptidase inhibitor determined free in solution and in complex with human carboxypeptidase A2., Reverter D, Fernandez-Catalan C, Baumgartner R, Pfander R, Huber R, Bode W, Vendrell J, Holak TA, Aviles FX, Nat Struct Biol. 2000 Apr;7(4):322-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10742178 10742178]
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Role of kinetic intermediates in the folding of leech carboxypeptidase inhibitor., Arolas JL, Bronsoms S, Lorenzo J, Aviles FX, Chang JY, Ventura S, J Biol Chem. 2004 Sep 3;279(36):37261-70. Epub 2004 Jun 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15226306 15226306]
[[Category: Hirudo medicinalis]]
[[Category: Hirudo medicinalis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Four-stranded antiparallel beta-sheet]]
[[Category: Four-stranded antiparallel beta-sheet]]
[[Category: Oxidative folding]]
[[Category: Oxidative folding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:33:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 01:41:02 2008''

Revision as of 22:41, 27 July 2008

Image:1zfl.png

Template:STRUCTURE 1zfl

Solution structure of III-A, the major intermediate in the oxidative folding of leech carboxypeptidase inhibitor

Template:ABSTRACT PUBMED 16084391

About this Structure

1ZFL is a Single protein structure of sequence from Hirudo medicinalis. Full experimental information is available from OCA.

Reference

NMR structural characterization and computational predictions of the major intermediate in oxidative folding of leech carboxypeptidase inhibitor., Arolas JL, D'Silva L, Popowicz GM, Aviles FX, Holak TA, Ventura S, Structure. 2005 Aug;13(8):1193-202. PMID:16084391

Structure of a novel leech carboxypeptidase inhibitor determined free in solution and in complex with human carboxypeptidase A2., Reverter D, Fernandez-Catalan C, Baumgartner R, Pfander R, Huber R, Bode W, Vendrell J, Holak TA, Aviles FX, Nat Struct Biol. 2000 Apr;7(4):322-8. PMID:10742178

Role of kinetic intermediates in the folding of leech carboxypeptidase inhibitor., Arolas JL, Bronsoms S, Lorenzo J, Aviles FX, Chang JY, Ventura S, J Biol Chem. 2004 Sep 3;279(36):37261-70. Epub 2004 Jun 28. PMID:15226306

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