1l1n
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1l1n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l1n, resolution 2.1Å" /> '''POLIOVIRUS 3C PROTEIN...)
Next diff →
Revision as of 18:03, 20 November 2007
|
POLIOVIRUS 3C PROTEINASE
Overview
The X-ray crystallographic structure of the recombinant poliovirus 3C gene, product (Mahoney strain) has been determined by single isomorphous, replacement and non-crystallographic symmetry averaging and refined at 2.1, A resolution. Poliovirus 3C is comprised of two six-stranded antiparallel, beta-barrel domains and is structurally similar to the chymotrypsin-like, serine proteinases. The shallow active site cleft is located at the, junction of the two beta-barrel domains and contains a His40, Glu71, Cys147 catalytic triad. The polypeptide loop preceding Cys147 is flexible, and likely undergoes a conformational change upon substrate binding. The, specificity pockets for poliovirus 3C are well-defined and modeling, studies account for the known substrate specificity of this proteinase., Poliovirus 3C also participates in the formation of the viral replicative, initiation complex where it specifically recognizes and binds the RNA, stem-loop structure in the 5' non-translated region of its own genome. The, RNA recognition site of 3C is located on the opposite side of the molecule, in relation to its proteolytic active site and is centered about the, conserved KFRDIR sequence of the domain linker. The recognition site is, well-defined and also includes residues from the amino and, carboxy-terminal helices. The two molecules in the asymmetric unit are, related by an approximate 2-fold, non-crystallographic symmetry and form, an intermolecular antiparallel beta-sheet at their interface.
About this Structure
1L1N is a Single protein structure of sequence from Human poliovirus 1. Active as Picornain 3C, with EC number 3.4.22.28 Full crystallographic information is available from OCA.
Reference
Refined X-ray crystallographic structure of the poliovirus 3C gene product., Mosimann SC, Cherney MM, Sia S, Plotch S, James MN, J Mol Biol. 1997 Nov 14;273(5):1032-47. PMID:9367789
Page seeded by OCA on Tue Nov 20 20:10:14 2007
