283d

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{{STRUCTURE_283d| PDB=283d | SCENE= }}
{{STRUCTURE_283d| PDB=283d | SCENE= }}
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'''A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING'''
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===A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING===
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==Overview==
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The crystal structure of the RNA dodecamer 5'-GGCC(GAAA)GGCC-3' has been determined from x-ray diffraction data to 2.3-A resolution. In the crystal, these oligomers form double helices around twofold symmetry axes. Four consecutive non-Watson-Crick base pairs make up an internal loop in the middle of the duplex, including sheared G.A pairs and novel asymmetric A.A pairs. This internal loop sequence produces a significant curvature and narrowing of the double helix. The helix is curved by 34 degrees from end to end and the diameter is narrowed by 24% in the internal loop. A Mn2+ ion is bound directly to the N7 of the first guanine in the Watson-Crick region following the internal loop and the phosphate of the preceding residue. This Mn2+ location corresponds to a metal binding site observed in the hammerhead catalytic RNA.
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(as it appears on PubMed at http://www.pubmed.gov), where 8917508 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8917508}}
==About this Structure==
==About this Structure==
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[[Category: Mismatched]]
[[Category: Mismatched]]
[[Category: Unusual rna]]
[[Category: Unusual rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:25:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:03:58 2008''

Revision as of 23:04, 27 July 2008

Image:283d.png

Template:STRUCTURE 283d

A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING

Template:ABSTRACT PUBMED 8917508

About this Structure

Full crystallographic information is available from OCA.

Reference

A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing., Baeyens KJ, De Bondt HL, Pardi A, Holbrook SR, Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12851-5. PMID:8917508

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