2npx

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{{STRUCTURE_2npx| PDB=2npx | SCENE= }}
{{STRUCTURE_2npx| PDB=2npx | SCENE= }}
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'''NADH BINDING SITE AND CATALYSIS OF NADH PEROXIDASE'''
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===NADH BINDING SITE AND CATALYSIS OF NADH PEROXIDASE===
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==Overview==
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The structure of the complex between cofactor NADH and the enzyme NADH peroxidase from Streptococcus faecalis 10C1 (Enterococcus faecalis) has been determined by crystal soaking, X-ray data collection, model building of NADH and refinement at 0.24-nm resolution based on the known enzyme structure [Stehle, T., Ahmed, S. A., Claiborne, A. &amp; Schulz, G. E. (1991) J. Mol. Biol. 221, 1325-1344]. Apart from NADH, the catalytic center of the enzyme contains FAD and a cysteine that shuttles between thiolate and sulfenic acid states. Unfortunately, this cysteine was irreversibly oxidized to a cysteine sulfonic acid in the established enzyme structure. Based on the geometry of the catalytic center, we discuss the stabilization of the oxidation-sensitive sulfenic acid and propose a reaction mechanism.
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(as it appears on PubMed at http://www.pubmed.gov), where 8425532 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8425532}}
==About this Structure==
==About this Structure==
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[[Category: Schulz, G E.]]
[[Category: Schulz, G E.]]
[[Category: Stehle, T.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:13:36 2008''

Revision as of 23:13, 27 July 2008

Image:2npx.png

Template:STRUCTURE 2npx

NADH BINDING SITE AND CATALYSIS OF NADH PEROXIDASE

Template:ABSTRACT PUBMED 8425532

About this Structure

2NPX is a Single protein structure of sequence from Enterococcus faecalis. Full crystallographic information is available from OCA.

Reference

NADH binding site and catalysis of NADH peroxidase., Stehle T, Claiborne A, Schulz GE, Eur J Biochem. 1993 Jan 15;211(1-2):221-6. PMID:8425532

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