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2nn8

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{{STRUCTURE_2nn8| PDB=2nn8 | SCENE= }}
{{STRUCTURE_2nn8| PDB=2nn8 | SCENE= }}
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'''Crystal structure of human galectin-3 carbohydrate-recognition domain with lactose bound, at 1.35 angstrom resolution'''
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===Crystal structure of human galectin-3 carbohydrate-recognition domain with lactose bound, at 1.35 angstrom resolution===
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==Overview==
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Galectin-3 is a multifunctional carbohydrate-binding protein that has roles in cancer progression. In addition to carbohydrate-dependent extracellular functions, galectin-3 participates in carbohydrate-independent intracellular signalling pathways, including apoptosis, via protein-protein interactions, some of which engage the carbohydrate-binding groove. When ligands bind within this site, conformational rearrangements are induced and information on unliganded galectin-3 is therefore valuable for structure-based drug design. Removal of cocrystallized lactose from the human galectin-3 carbohydrate-recognition domain was achieved via crystal soaking, but took weeks despite low affinity. Pre-soaking to remove lactose enabled the subsequent binding of cryoprotectant glycerol, whereas when the lactose was not removed a priori the glycerol could not displace it in the short cryosoaking time frame. This slow diffusion of lactose out of the crystals contrasts with the entrance of glycerol, which takes place within minutes. The importance of the removal of incumbent ligands prior to attempts to introduce alternative ligands is indicated, even for proteins exhibiting low affinity for ligands, and has significance for ligand exchange in structure-based drug design.
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(as it appears on PubMed at http://www.pubmed.gov), where 17327679 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17327679}}
==About this Structure==
==About this Structure==
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[[Category: Collins, P M.]]
[[Category: Collins, P M.]]
[[Category: Beta-sandwich]]
[[Category: Beta-sandwich]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:39:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:15:58 2008''

Revision as of 23:16, 27 July 2008

Image:2nn8.png

Template:STRUCTURE 2nn8

Crystal structure of human galectin-3 carbohydrate-recognition domain with lactose bound, at 1.35 angstrom resolution

Template:ABSTRACT PUBMED 17327679

About this Structure

2NN8 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Slow diffusion of lactose out of galectin-3 crystals monitored by X-ray crystallography: possible implications for ligand-exchange protocols., Collins PM, Hidari KI, Blanchard H, Acta Crystallogr D Biol Crystallogr. 2007 Mar;63(Pt 3):415-9. Epub 2007, Feb 21. PMID:17327679

Page seeded by OCA on Mon Jul 28 02:15:58 2008

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