2hf8

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{{STRUCTURE_2hf8| PDB=2hf8 | SCENE= }}
{{STRUCTURE_2hf8| PDB=2hf8 | SCENE= }}
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'''Crystal structure of HypB from Methanocaldococcus jannaschii in the triphosphate form, in complex with zinc'''
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===Crystal structure of HypB from Methanocaldococcus jannaschii in the triphosphate form, in complex with zinc===
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==Overview==
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HypB is a prokaryotic metal-binding guanine nucleotide-binding protein that is essential for nickel incorporation into hydrogenases. Here we solved the x-ray structure of HypB from Methanocaldococcus jannaschii. It shows that the G-domain has a different topology than the Ras-like proteins and belongs to the SIMIBI (after Signal Recognition Particle, MinD and BioD) class of NTP-binding proteins. We show that HypB undergoes nucleotide-dependent dimerization, which is apparently a common feature of SIMIBI class G-proteins. The nucleotides are located in the dimer interface and are contacted by both subunits. The active site features residues from both subunits arguing that hydrolysis also requires dimerization. Two metal-binding sites are found, one of which is dependent on the state of bound nucleotide. A totally conserved ENV/IGNLV/ICP motif in switch II relays the nucleotide binding with the metal ionbinding site. The homology with NifH, the Fe protein subunit of nitrogenase, suggests a mechanistic model for the switch-dependent incorporation of a metal ion into hydrogenases.
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{{ABSTRACT_PUBMED_16807243}}
==About this Structure==
==About this Structure==
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[[Category: Alpha and beta protein]]
[[Category: Alpha and beta protein]]
[[Category: P-loop containing nucleoside triphosphate hydrolase]]
[[Category: P-loop containing nucleoside triphosphate hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:13:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:24:40 2008''

Revision as of 23:24, 27 July 2008

Image:2hf8.png

Template:STRUCTURE 2hf8

Crystal structure of HypB from Methanocaldococcus jannaschii in the triphosphate form, in complex with zinc

Template:ABSTRACT PUBMED 16807243

About this Structure

2HF8 is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.

Reference

Structural insights into HypB, a GTP-binding protein that regulates metal binding., Gasper R, Scrima A, Wittinghofer A, J Biol Chem. 2006 Sep 15;281(37):27492-502. Epub 2006 Jun 28. PMID:16807243

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