2o7l

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[[Image:2o7l.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_2o7l| PDB=2o7l | SCENE= }}
{{STRUCTURE_2o7l| PDB=2o7l | SCENE= }}
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'''The open-cap conformation of GlpG'''
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===The open-cap conformation of GlpG===
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==Overview==
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The active sites of intramembrane proteases are positioned in the lipid bilayer to facilitate peptide bond hydrolysis in the membrane. Previous crystallographic analysis of Escherichia coli GlpG, an intramembrane protease of the rhomboid family, has revealed an internal and hydrophilic active site in an apparently closed conformation. Here we describe the crystal structure of GlpG in a more open conformation, where the capping loop L5 has been lifted, exposing the previously buried and catalytically essential Ser-201 to outside aqueous solution. A water molecule now moves into the putative oxyanion hole that is constituted of a main-chain amide (Ser-201) and two conserved side chains (His-150 and Asn-154). The loop movement also destabilizes a hydrophobic side chain (Phe-245) previously buried between transmembrane helices S2 and S5 and creates a side portal from the lipid to protease active site. These results provide insights into the conformational plasticity of GlpG to accommodate substrate binding and catalysis and into the chirality of the reaction intermediate.
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(as it appears on PubMed at http://www.pubmed.gov), where 17277078 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17277078}}
==About this Structure==
==About this Structure==
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[[Category: Membrane protein]]
[[Category: Membrane protein]]
[[Category: Rhomboid protease]]
[[Category: Rhomboid protease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:25:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:26:58 2008''

Revision as of 23:27, 27 July 2008

Image:2o7l.png

Template:STRUCTURE 2o7l

The open-cap conformation of GlpG

Template:ABSTRACT PUBMED 17277078

About this Structure

2O7L is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Open-cap conformation of intramembrane protease GlpG., Wang Y, Ha Y, Proc Natl Acad Sci U S A. 2007 Feb 13;104(7):2098-102. Epub 2007 Feb 2. PMID:17277078

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