2c84

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{{STRUCTURE_2c84| PDB=2c84 | SCENE= }}
{{STRUCTURE_2c84| PDB=2c84 | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE SIALYLTRANSFERASE PM0188 WITH CMP'''
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===CRYSTAL STRUCTURE OF THE SIALYLTRANSFERASE PM0188 WITH CMP===
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==Overview==
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PM0188 is a newly identified sialyltransferase from P. multocida which transfers sialic acid from cytidine 5'-monophosphonuraminic acid (CMP-NeuAc) to an acceptor sugar. Although sialyltransferases are involved in important biological functions like cell-cell recognition, cell differentiation and receptor-ligand interactions, little is known about their catalytic mechanism. Here, we report the X-ray crystal structures of PM0188 in the presence of an acceptor sugar and a donor sugar analogue, revealing the precise mechanism of sialic acid transfer. Site-directed mutagenesis, kinetic assays, and structural analysis show that Asp141, His311, Glu338, Ser355 and Ser356 are important catalytic residues; Asp141 is especially crucial as it acts as a general base. These complex structures provide insights into the mechanism of sialyltransferases and the structure-based design of specific inhibitors.
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(as it appears on PubMed at http://www.pubmed.gov), where 18304450 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18304450}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structural analysis of sialyltransferase PM0188 from Pasteurella multocida complexed with donor analogue and acceptor sugar., Kim DU, Yoo JH, Lee YJ, Kim KS, Cho HS, BMB Rep. 2008 Jan 31;41(1):48-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18304450 18304450]
Structural analysis of sialyltransferase PM0188 from Pasteurella multocida complexed with donor analogue and acceptor sugar., Kim DU, Yoo JH, Lee YJ, Kim KS, Cho HS, BMB Rep. 2008 Jan 31;41(1):48-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18304450 18304450]
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Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog., Chiu CP, Watts AG, Lairson LL, Gilbert M, Lim D, Wakarchuk WW, Withers SG, Strynadka NC, Nat Struct Mol Biol. 2004 Feb;11(2):163-70. Epub 2004 Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14730352 14730352]
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Crystallization and preliminary X-ray crystallographic analysis of the alpha-2,6-sialyltransferase PM0188 from Pasteurella multosida., Kim DU, Yoo JH, Ryu K, Cho HS, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Feb 1;62(Pt, 2):142-4. Epub 2006 Jan 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511286 16511286]
[[Category: Pasteurella multocida]]
[[Category: Pasteurella multocida]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Sialyltransferase]]
[[Category: Sialyltransferase]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:25:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:27:57 2008''

Revision as of 23:28, 27 July 2008

Image:2c84.png

Template:STRUCTURE 2c84

CRYSTAL STRUCTURE OF THE SIALYLTRANSFERASE PM0188 WITH CMP

Template:ABSTRACT PUBMED 18304450

About this Structure

2C84 is a Single protein structure of sequence from Pasteurella multocida. Full crystallographic information is available from OCA.

Reference

Structural analysis of sialyltransferase PM0188 from Pasteurella multocida complexed with donor analogue and acceptor sugar., Kim DU, Yoo JH, Lee YJ, Kim KS, Cho HS, BMB Rep. 2008 Jan 31;41(1):48-54. PMID:18304450

Structural analysis of the sialyltransferase CstII from Campylobacter jejuni in complex with a substrate analog., Chiu CP, Watts AG, Lairson LL, Gilbert M, Lim D, Wakarchuk WW, Withers SG, Strynadka NC, Nat Struct Mol Biol. 2004 Feb;11(2):163-70. Epub 2004 Jan 18. PMID:14730352

Crystallization and preliminary X-ray crystallographic analysis of the alpha-2,6-sialyltransferase PM0188 from Pasteurella multosida., Kim DU, Yoo JH, Ryu K, Cho HS, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Feb 1;62(Pt, 2):142-4. Epub 2006 Jan 27. PMID:16511286

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