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2f5x

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{{STRUCTURE_2f5x| PDB=2f5x | SCENE= }}
{{STRUCTURE_2f5x| PDB=2f5x | SCENE= }}
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'''Structure of periplasmic binding protein BugD'''
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===Structure of periplasmic binding protein BugD===
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==Overview==
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Periplasmic binding proteins of a new family particularly well represented in Bordetella pertussis have been called Bug receptors. One B.pertussis Bug protein is part of a tripartite tricarboxylate transporter while the functions of the other 77 are unknown. We report the first structure of a Bug receptor, BugD. It adopts the characteristic Venus flytrap motif observed in other periplasmic binding proteins, with two globular domains bisected by a deep cleft. BugD displays a closed conformation resulting from the fortuitous capture of a ligand, identified from the electron density as an aspartate. The structure reveals a distinctive alpha carboxylate-binding motif, involving two water molecules that bridge the carboxylate oxygen atoms to the protein. Both water molecules are hydrogen bonded to a common carbonyl group from Ala14, and each forms a hydrogen bond with one carboxylate oxygen atom of the ligand. Additional hydrogen bonds are found between the ligand alpha carboxylate oxygen atoms and protein backbone amide groups and with a threonine hydroxyl group. This specific ligand-binding motif is highly conserved in Bug proteins, indicating that they may all be receptors of amino acids or other carboxylated solutes, with a similar binding mode. The present structure thus unveils the bases of ligand binding in this large family of periplasmic binding proteins, several hundred members of which have been identified in various bacterial species.
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{{ABSTRACT_PUBMED_16403514}}
==About this Structure==
==About this Structure==
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[[Category: Villeret, V.]]
[[Category: Villeret, V.]]
[[Category: Periplasmic binding protein]]
[[Category: Periplasmic binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:30:18 2008''

Revision as of 23:30, 27 July 2008

Image:2f5x.png

Template:STRUCTURE 2f5x

Structure of periplasmic binding protein BugD

Template:ABSTRACT PUBMED 16403514

About this Structure

2F5X is a Single protein structure of sequence from Bordetella pertussis tohama i. Full crystallographic information is available from OCA.

Reference

Crystal structure of Bordetella pertussis BugD solute receptor unveils the basis of ligand binding in a new family of periplasmic binding proteins., Huvent I, Belrhali H, Antoine R, Bompard C, Locht C, Jacob-Dubuisson F, Villeret V, J Mol Biol. 2006 Mar 3;356(4):1014-26. Epub 2005 Dec 21. PMID:16403514

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