2d1x

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[[Image:2d1x.gif|left|200px]]
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{{Seed}}
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[[Image:2d1x.png|left|200px]]
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{{STRUCTURE_2d1x| PDB=2d1x | SCENE= }}
{{STRUCTURE_2d1x| PDB=2d1x | SCENE= }}
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'''The crystal structure of the cortactin-SH3 domain and AMAP1-peptide complex'''
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===The crystal structure of the cortactin-SH3 domain and AMAP1-peptide complex===
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==Overview==
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Invasive potentials of carcinomas greatly contribute to their metastasis, which is a major threat in most cancers. We have recently shown that Arf6 plays a pivotal role in breast cancer invasive activities and identified AMAP1 as an effector of GTP-Arf6 in invasion. Expression of AMAP1 correlates well with invasive phenotypes of primary tumors of the human breast. We also have shown that AMAP1 functions by forming a trimeric protein complex with cortactin and paxillin. In this complex, AMAP1 binds to the src homology 3 (SH3) domain of cortactin via its proline-rich peptide, SKKRPPPPPPGHKRT. SH3 domains are known to bind generally to the proline-rich ligands with a one-to-one stoichiometry. We found that AMAP1/cortactin binding is very atypical in its stoichiometry and interface structure, in which one AMAP1 proline-rich peptide binds to two cortactin SH3 domains simultaneously. We made a cell-permeable peptide derived from the AMAP1 peptide, and we show that this peptide specifically blocks AMAP1/cortactin binding, but not other canonical SH3/proline bindings, and effectively inhibits breast cancer invasion and metastasis. Moreover, this peptide was found to block invasion of other types of cancers, such as glioblastomas and lung carcinomas. We also found that a small-molecule compound, UCS15A, which was previously judged as a weak inhibitor against canonical SH3/proline bindings, effectively inhibits AMAP1/cortactin binding and breast cancer invasion and metastasis. Together with fine structural analysis, we propose that the AMAP1/cortactin complex, which is not detected in normal mammary epithelial cells, is an excellent drug target for cancer therapeutics.
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(as it appears on PubMed at http://www.pubmed.gov), where 16636290 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16636290}}
==About this Structure==
==About this Structure==
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[[Category: Proline-rich]]
[[Category: Proline-rich]]
[[Category: Sh3]]
[[Category: Sh3]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:32:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:38:47 2008''

Revision as of 23:38, 27 July 2008

Image:2d1x.png

Template:STRUCTURE 2d1x

The crystal structure of the cortactin-SH3 domain and AMAP1-peptide complex

Template:ABSTRACT PUBMED 16636290

About this Structure

2D1X is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Targeting AMAP1 and cortactin binding bearing an atypical src homology 3/proline interface for prevention of breast cancer invasion and metastasis., Hashimoto S, Hirose M, Hashimoto A, Morishige M, Yamada A, Hosaka H, Akagi K, Ogawa E, Oneyama C, Agatsuma T, Okada M, Kobayashi H, Wada H, Nakano H, Ikegami T, Nakagawa A, Sabe H, Proc Natl Acad Sci U S A. 2006 May 2;103(18):7036-41. Epub 2006 Apr 24. PMID:16636290

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