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1p7l

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{{STRUCTURE_1p7l| PDB=1p7l | SCENE= }}
{{STRUCTURE_1p7l| PDB=1p7l | SCENE= }}
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'''S-Adenosylmethionine synthetase complexed with AMPPNP and Met.'''
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===S-Adenosylmethionine synthetase complexed with AMPPNP and Met.===
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==Overview==
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S-Adenosylmethionine synthetase (MAT) catalyzes formation of S-adenosylmethionine (SAM) from ATP and l-methionine (Met) and hydrolysis of tripolyphosphate to PP(i) and P(i). Escherichia coli MAT (eMAT) has been crystallized with the ATP analogue AMPPNP and Met, and the crystal structure has been determined at 2.5 A resolution. eMAT is a dimer of dimers and has a 222 symmetry. Each active site contains the products SAM and PPNP. A modeling study indicates that the substrates (AMPPNP and Met) can bind at the same sites as the products, and only a small conformation change of the ribose ring is needed for conversion of the substrates to the products. On the basis of the ternary complex structure and a modeling study, a novel catalytic mechanism of SAM formation is proposed. In the mechanism, neutral His14 acts as an acid to cleave the C5'-O5' bond of ATP while simultaneously a change in the ribose ring conformation from C4'-exo to C3'-endo occurs, and the S of Met makes a nucleophilic attack on the C5' to form SAM. All essential amino acid residues for substrate binding found in eMAT are conserved in the rat liver enzyme, indicating that the bacterial and mammalian enzymes have the same catalytic mechanism. However, a catalytic mechanism proposed recently by Gonzalez et al. based on the structures of three ternary complexes of rat liver MAT [Gonzalez, B., Pajares, M. A., Hermoso, J. A., Guillerm, D., Guillerm, G., and Sanz-Aparicio. J. (2003) J. Mol. Biol. 331, 407] is substantially different from our mechanism.
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(as it appears on PubMed at http://www.pubmed.gov), where 14967023 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14967023}}
==About this Structure==
==About this Structure==
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[[Category: S-adenosylmethionine synthetase]]
[[Category: S-adenosylmethionine synthetase]]
[[Category: Sam]]
[[Category: Sam]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:54:01 2008''

Revision as of 23:54, 27 July 2008

Image:1p7l.png

Template:STRUCTURE 1p7l

S-Adenosylmethionine synthetase complexed with AMPPNP and Met.

Template:ABSTRACT PUBMED 14967023

About this Structure

1P7L is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of the S-adenosylmethionine synthetase ternary complex: a novel catalytic mechanism of S-adenosylmethionine synthesis from ATP and Met., Komoto J, Yamada T, Takata Y, Markham GD, Takusagawa F, Biochemistry. 2004 Feb 24;43(7):1821-31. PMID:14967023

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