This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1tz4

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1tz4.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1tz4.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1tz4| PDB=1tz4 | SCENE= }}
{{STRUCTURE_1tz4| PDB=1tz4 | SCENE= }}
-
'''[hPP19-23]-pNPY bound to DPC Micelles'''
+
===[hPP19-23]-pNPY bound to DPC Micelles===
-
==Overview==
+
<!--
-
Neuropeptide Y (NPY) and the pancreatic polypeptide (PP) are members of the neuropeptide Y family of hormones. They bind to the Y receptors with very different affinities: Whereas PP is highly selective for the Y(4) receptor, NPY displays highest affinites for Y(1), Y(2), and Y(5) receptor subtypes. Introducing the NPY segment 19-23 into PP leads to an increase in affinity at the Y(1) and Y(2) receptor subtypes whereas the exchange of this segment from PP into NPY leads to a large decrease in affinity at all receptor subtypes. PP displays a very stable structure in solution, with the N terminus being back-folded onto the C-terminal alpha-helix (the so-called PP-fold). The helix of NPY is less stable and the N terminus is freely diffusing in solution. The exchange of this segment, however, does not alter the PP-fold propensities of the chimeric peptides in solution. The structures of the phospholipid micelle-bound peptides serving to mimic the membrane-bound species display segregation into a more flexible N-terminal region and a well-defined alpha-helical region. The introduction of the [19-23]-pNPY segment into hPP leads to an N-terminal extension of the alpha-helix, now starting at Pro(14) instead of Met(17). In contrast, a truncated helix is observed in [(19)(-)(23)hPP]-pNPY, starting at Leu(17) instead of Ala(14). All peptides display moderate binding affinities to neutral membranes (K(assoc) in the range of 1.7 to 6.8 x 10(4) mol(-)(1) as determined by surface plasmon resonance) with the differences in binding being most probably related to the exchange of Arg-19 (pNPY) by Glu-23 (hPP). Differences in receptor binding properties between the chimeras and their parental peptides are therefore most likely due to changes in the conformation of the micelle-bound peptides.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15966750}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15966750 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15966750}}
==About this Structure==
==About this Structure==
-
1TZ4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa_and_homo_sapiens Sus scrofa and homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZ4 OCA].
+
1TZ4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa_and_homo_sapiens Sus scrofa and homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZ4 OCA].
==Reference==
==Reference==
Line 29: Line 33:
[[Category: Zerbe, O.]]
[[Category: Zerbe, O.]]
[[Category: Npy-pp chimera]]
[[Category: Npy-pp chimera]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:32:41 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:01:18 2008''

Revision as of 00:01, 28 July 2008

Image:1tz4.png

Template:STRUCTURE 1tz4

[hPP19-23]-pNPY bound to DPC Micelles

Template:ABSTRACT PUBMED 15966750

About this Structure

1TZ4 is a Single protein structure of sequence from Sus scrofa and homo sapiens. Full experimental information is available from OCA.

Reference

Strongly altered receptor binding properties in PP and NPY chimeras are accompanied by changes in structure and membrane binding., Lerch M, Kamimori H, Folkers G, Aguilar MI, Beck-Sickinger AG, Zerbe O, Biochemistry. 2005 Jun 28;44(25):9255-64. PMID:15966750

Page seeded by OCA on Mon Jul 28 03:01:18 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1tz4"
Personal tools