1n0i

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{{STRUCTURE_1n0i| PDB=1n0i | SCENE= }}
{{STRUCTURE_1n0i| PDB=1n0i | SCENE= }}
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'''Crystal Structure of Ferrochelatase with Cadmium bound at active site'''
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===Crystal Structure of Ferrochelatase with Cadmium bound at active site===
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==Overview==
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Ferrochelatase, the terminal enzyme in heme biosynthesis, catalyses metal insertion into protoporphyrin IX. The location of the metal binding site with respect to the bound porphyrin substrate and the mode of metal binding are of central importance for understanding the mechanism of porphyrin metallation. In this work we demonstrate that Zn(2+), which is commonly used as substrate in assays of the ferrochelatase reaction, and Cd(2+), an inhibitor of the enzyme, bind to the invariant amino acids His183 and Glu264 and water molecules, all located within the porphyrin binding cleft. On the other hand, Mg(2+), which has been shown to bind close to the surface at 7 A from His183, was largely absent from its site. Activity measurements demonstrate that Mg(2+) has a stimulatory effect on the enzyme, lowering K(M) for Zn(2+) from 55 to 24 micro M. Changing one of the Mg(2+) binding residues, Glu272, to serine abolishes the effect of Mg(2+). It is proposed that prior to metal insertion the metal may form a sitting-atop (SAT) complex with the invariant His-Glu couple and the porphyrin. Metal binding to the Mg(2+) site may stimulate metal release from the protein ligands and its insertion into the porphyrin.
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(as it appears on PubMed at http://www.pubmed.gov), where 12761666 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12761666}}
==About this Structure==
==About this Structure==
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[[Category: Sigfridsson, E.]]
[[Category: Sigfridsson, E.]]
[[Category: Pi-helix]]
[[Category: Pi-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:56:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:10:09 2008''

Revision as of 00:10, 28 July 2008

Image:1n0i.png

Template:STRUCTURE 1n0i

Crystal Structure of Ferrochelatase with Cadmium bound at active site

Template:ABSTRACT PUBMED 12761666

About this Structure

1N0I is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Metal binding to Bacillus subtilis ferrochelatase and interaction between metal sites., Lecerof D, Fodje MN, Alvarez Leon R, Olsson U, Hansson A, Sigfridsson E, Ryde U, Hansson M, Al-Karadaghi S, J Biol Inorg Chem. 2003 Apr;8(4):452-8. Epub 2003 Jan 18. PMID:12761666

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