1zfs

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[[Image:1zfs.gif|left|200px]]
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{{STRUCTURE_1zfs| PDB=1zfs | SCENE= }}
{{STRUCTURE_1zfs| PDB=1zfs | SCENE= }}
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'''Solution structure of S100A1 bound to calcium'''
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===Solution structure of S100A1 bound to calcium===
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==Overview==
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S100A1 is an EF-hand-containing Ca(2+)-binding protein that undergoes a conformational change upon binding calcium as is necessary to interact with protein targets and initiate a biological response. To better understand how calcium influences the structure and function of S100A1, the three-dimensional structure of calcium-bound S100A1 was determined by multidimensional NMR spectroscopy and compared to the previously determined structure of apo. In total, 3354 nuclear Overhauser effect-derived distance constraints, 240 dihedral constraints, 160 hydrogen bond constraints, and 362 residual dipolar coupling restraints derived from a series of two-dimensional, three-dimensional, and four-dimensional NMR experiments were used in its structure determination (&gt;21 constraints per residue). As with other dimeric S100 proteins, S100A1 is a symmetric homodimer with helices 1, 1', 4, and 4' associating into an X-type four-helix bundle at the dimer interface. Within each subunit there are four alpha-helices and a short antiparallel beta-sheet typical of two helix-loop-helix EF-hand calcium-binding domains. The addition of calcium did not change the interhelical angle of helices 1 and 2 in the pseudo EF-hand significantly; however, there was a large reorientation of helix 3 in the typical EF-hand. The large conformational change exposes a hydrophobic cleft, defined by residues in the hinge region, the C terminus, and regions of helix 3, which are important for the interaction between S100A1 and a peptide (TRTK-12) derived from the actin-capping protein CapZ.
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{{ABSTRACT_PUBMED_16169012}}
==About this Structure==
==About this Structure==
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1ZFS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZFS OCA].
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1ZFS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZFS OCA].
==Reference==
==Reference==
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[[Category: S100]]
[[Category: S100]]
[[Category: X-type 4 helix bundle]]
[[Category: X-type 4 helix bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:34:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:16:13 2008''

Revision as of 00:16, 28 July 2008

Image:1zfs.png

Template:STRUCTURE 1zfs

Solution structure of S100A1 bound to calcium

Template:ABSTRACT PUBMED 16169012

About this Structure

1ZFS is a Single protein structure of sequence from Rattus norvegicus. Full experimental information is available from OCA.

Reference

The three-dimensional solution structure of Ca(2+)-bound S100A1 as determined by NMR spectroscopy., Wright NT, Varney KM, Ellis KC, Markowitz J, Gitti RK, Zimmer DB, Weber DJ, J Mol Biol. 2005 Oct 21;353(2):410-26. PMID:16169012

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