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1ywa

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{{STRUCTURE_1ywa| PDB=1ywa | SCENE= }}
{{STRUCTURE_1ywa| PDB=1ywa | SCENE= }}
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'''0.9 A Structure of NP4 from Rhodnius Prolixus complexed with CO at pH 5.6'''
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===0.9 A Structure of NP4 from Rhodnius Prolixus complexed with CO at pH 5.6===
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==Overview==
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Nitrophorin 4 (NP4), a nitric oxide (NO)-transport protein from the blood-sucking insect Rhodnius prolixus, uses a ferric (Fe3+) heme to deliver NO to its victims. NO binding to NP4 induces a large conformational change and complete desolvation of the distal pocket. The heme is markedly nonplanar, displaying a ruffling distortion postulated to contribute to stabilization of the ferric iron. Here, we report the ferrous (Fe2+) complexes of NP4 with NO, CO, and H2O formed after chemical reduction of the protein and the characterization of these complexes by absorption spectroscopy, flash photolysis, and ultrahigh-resolution crystallography (resolutions vary from 0.9 to 1.08 A). The absorption spectra, both in solution and in the crystal, are typical for six-coordinated ferrous complexes. Closure and desolvation of the distal pocket occurs upon binding CO or NO to the iron regardless of the heme oxidation state, confirming that the conformational change is driven by distal ligand polarity. The degree of heme ruffling is coupled to the nature of the ligand and the iron oxidation state in the following order: (Fe3+)-NO &gt; (Fe2+)-NO &gt; (Fe2+)-CO &gt; (Fe3+)-H2O &gt; (Fe2+)-H2O. The ferrous coordination geometry is as expected, except for the proximal histidine bond, which is shorter than typically found in model compounds. These data are consistent with heme ruffling and coordination geometry serving to stabilize the ferric state of the nitrophorins, a requirement for their physiological function. Possible roles for heme distortion and NO bending in heme protein function are discussed.
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(as it appears on PubMed at http://www.pubmed.gov), where 16171383 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16171383}}
==About this Structure==
==About this Structure==
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[[Category: Ferrous heme]]
[[Category: Ferrous heme]]
[[Category: Lipocalin fold]]
[[Category: Lipocalin fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:52:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:19:11 2008''

Revision as of 00:19, 28 July 2008

Image:1ywa.png

Template:STRUCTURE 1ywa

0.9 A Structure of NP4 from Rhodnius Prolixus complexed with CO at pH 5.6

Template:ABSTRACT PUBMED 16171383

About this Structure

1YWA is a Single protein structure of sequence from Rhodnius prolixus. Full crystallographic information is available from OCA.

Reference

Ultrahigh resolution structures of nitrophorin 4: heme distortion in ferrous CO and NO complexes., Maes EM, Roberts SA, Weichsel A, Montfort WR, Biochemistry. 2005 Sep 27;44(38):12690-9. PMID:16171383

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