1ps3

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{{STRUCTURE_1ps3| PDB=1ps3 | SCENE= }}
{{STRUCTURE_1ps3| PDB=1ps3 | SCENE= }}
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'''Golgi alpha-mannosidase II in complex with kifunensine'''
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===Golgi alpha-mannosidase II in complex with kifunensine===
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==Overview==
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Mannosidases are key enzymes in the eukaryotic N-glycosylation pathway. These enzymes fall into two broad classes (I and II) and are characteristically different in catalytic mechanism, sequence, and structure. Kifunensine is an alkaloid that is a strong inhibitor against class I alpha-mannosidases but is only a weak inhibitor against class II alpha-mannosidases. In this paper, the 1.80 A resolution crystal structure of kifunensine bound to Drosophila melanogaster Golgi alpha-mannosidase II (dGMII) is presented. Kifunensine adopts a (1,4)B boat conformation in the class II dGMII, which contrasts the (1)C(4) chair conformation seen in class I human endoplasmic reticulum alpha1,2 mannosidase (hERMI, PDB ). The observed conformations are higher in conformational energy than the global minimum (4)C(1) conformation, although the conformation in hERMI is closer to the minimum, as supported by an energy calculation. Differing conformations of 1-deoxymannojirimycin were also observed: a (4)C(1) and (1)C(4) conformation in dGMII and hERMI, respectively. Thus, these two alpha-mannosidase classes distort these inhibitors in distinct manners. This is likely indicative of the binding characteristics of the two different catalytic mechanisms of these enzymes.
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{{ABSTRACT_PUBMED_14636047}}
==About this Structure==
==About this Structure==
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[[Category: N-terminal alpha-beta domain]]
[[Category: N-terminal alpha-beta domain]]
[[Category: Three helix bundle]]
[[Category: Three helix bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:26:05 2008''

Revision as of 00:26, 28 July 2008

Image:1ps3.png

Template:STRUCTURE 1ps3

Golgi alpha-mannosidase II in complex with kifunensine

Template:ABSTRACT PUBMED 14636047

About this Structure

1PS3 is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

Reference

Comparison of kifunensine and 1-deoxymannojirimycin binding to class I and II alpha-mannosidases demonstrates different saccharide distortions in inverting and retaining catalytic mechanisms., Shah N, Kuntz DA, Rose DR, Biochemistry. 2003 Dec 2;42(47):13812-6. PMID:14636047

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