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1w52

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{{STRUCTURE_1w52| PDB=1w52 | SCENE= }}
{{STRUCTURE_1w52| PDB=1w52 | SCENE= }}
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'''CRYSTAL STRUCTURE OF A PROTEOLYZED FORM OF PANCREATIC LIPASE RELATED PROTEIN 2 FROM HORSE'''
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===CRYSTAL STRUCTURE OF A PROTEOLYZED FORM OF PANCREATIC LIPASE RELATED PROTEIN 2 FROM HORSE===
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==Overview==
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Horse pancreatic lipase-related proteins PLRP1 and PLRP2 are produced by the pancreas together with pancreatic lipase (PL). Sequence-comparison analyses reveal that the three proteins possess the same two-domain organization: an N-terminal catalytic domain and a C-terminal domain, which in PL is involved in colipase binding. Nevertheless, despite the high level of sequence identity found, they exhibit distinct enzymatic properties. The intrinsic sensitivity of the peptide bond between Ser245 and Thr246 within the flap region of PLRP2 to proteolytic cleavage probably complicates PLRP2 crystallization since, as shown here, this proteolyzed form of PLRP2 is only crystallized after specific detergent stabilization of this region. This has been performed by the hanging-drop vapour-diffusion method at 291 K and exclusively in the presence of N,N-dimethyldecylamine-beta-oxide (DDAO). However, most crystals (&gt;95%) are highly twinned and diffract poorly (to approximately 7-5 A resolution). Diffraction-quality trigonal crystals have unit-cell parameters a = b = 128.4, c = 85.8 A and belong to space group P3(2)21. A 2.9 A native data set was collected at ESRF on beamline ID14-2 with an R(merge) of 12.7%. Preliminary structural analysis provides a structural basis for the specific roles of DDAO.
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{{ABSTRACT_PUBMED_15502342}}
==About this Structure==
==About this Structure==
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[[Category: Lipase]]
[[Category: Lipase]]
[[Category: Pancreatic lipase related protein]]
[[Category: Pancreatic lipase related protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:30:19 2008''

Revision as of 00:30, 28 July 2008

Image:1w52.png

Template:STRUCTURE 1w52

CRYSTAL STRUCTURE OF A PROTEOLYZED FORM OF PANCREATIC LIPASE RELATED PROTEIN 2 FROM HORSE

Template:ABSTRACT PUBMED 15502342

About this Structure

1W52 is a Single protein structure of sequence from Equus caballus. Full crystallographic information is available from OCA.

Reference

Crystallization of a proteolyzed form of the horse pancreatic lipase-related protein 2: structural basis for the specific detergent requirement., Mancheno JM, Jayne S, Kerfelec B, Chapus C, Crenon I, Hermoso JA, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2107-9. Epub 2004, Oct 20. PMID:15502342

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