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| | {{STRUCTURE_1nbl| PDB=1nbl | SCENE= }} | | {{STRUCTURE_1nbl| PDB=1nbl | SCENE= }} |
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| - | '''NMR Structure of Hellethionin D'''
| + | ===NMR Structure of Hellethionin D=== |
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| - | ==Overview==
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| - | Thionins are relatively small-sized multiple-cystine peptides that are probably involved in the plant defense against pathogens. As such, these peptides constitute promising candidates for engineered plant resistance in the agricultural industry. More recently, thionins have been proposed as potential immunotoxins in tumor therapy. In the search for pharmacologically active natural products, a new family of thionins was recently discovered in the roots of Helleborus purpurascens that accordingly were termed hellethionins. The structural characterization by NMR of one representative member of this family, i.e., of hellethionin D, clearly reveals that thionins from different sources share a highly conserved overall fold. In fact, the well-defined 3D structure of hellethionin D is very similar to those reported so far for viscotoxins, purothionins, or crambin, although distinct differences could be detected in the C-terminal portion, especially for loop 36-39. These differences may derive from the unusual distribution of charged residues in the C-terminal half of the peptide sequence compared to other thionins and from the uncommon occurrence of four contiguous threonine residues in loop 36-39. As expected, reduction of the disulfide bonds in hellethionin D leads to complete unfolding, but upon oxidative refolding by air oxygen in the presence of glutathione the correct isomer is recovered in high yields, confirming the very robust fold of this class of bioactive cystine peptides.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_12600207}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 12600207 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_12600207}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBL OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBL OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Renner, C.]] | | [[Category: Renner, C.]] |
| | [[Category: Gamma thionins helleborus]] | | [[Category: Gamma thionins helleborus]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:20:08 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:31:43 2008'' |
Revision as of 00:31, 28 July 2008
Template:STRUCTURE 1nbl
NMR Structure of Hellethionin D
Template:ABSTRACT PUBMED 12600207
About this Structure
Full experimental information is available from OCA.
Reference
Structural characterization of hellethionins from Helleborus purpurascens., Milbradt AG, Kerek F, Moroder L, Renner C, Biochemistry. 2003 Mar 4;42(8):2404-11. PMID:12600207
Page seeded by OCA on Mon Jul 28 03:31:43 2008
