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2p8r

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{{STRUCTURE_2p8r| PDB=2p8r | SCENE= }}
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'''Crystal structure of the C-terminal domain of C. elegans pre-mRNA splicing factor Prp8 carrying R2303K mutant'''
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===Crystal structure of the C-terminal domain of C. elegans pre-mRNA splicing factor Prp8 carrying R2303K mutant===
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==Overview==
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Prp8 is a critical pre-mRNA splicing factor. Prp8 is proposed to help form and stabilize the spliceosome catalytic core and to be an important regulator of spliceosome activation. Mutations in human Prp8 (hPrp8) cause a severe form of the genetic disorder retinitis pigmentosa, RP13. Understanding the molecular mechanism of Prp8's function in pre-mRNA splicing and RP13 has been hindered by its large size (over 2000 amino acids) and remarkably low-sequence similarity with other proteins. Here we present the crystal structure of the C-terminal domain (the last 273 residues) of Caenorhabditis elegans Prp8 (cPrp8). The core of the C-terminal domain is an alpha/beta structure that forms the MPN (Mpr1, Pad1 N-terminal) fold but without Zn(2+) coordination. We propose that the C-terminal domain is a protein interaction domain instead of a Zn(2+)-dependent metalloenzyme as proposed for some MPN proteins. Mapping of RP13 mutants on the Prp8 structure suggests that these residues constitute a binding surface between Prp8 and other partner(s), and the disruption of this interaction provides a plausible molecular mechanism for RP13.
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(as it appears on PubMed at http://www.pubmed.gov), where 17473007 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17473007}}
==About this Structure==
==About this Structure==
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[[Category: Splicing]]
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Revision as of 00:33, 28 July 2008

Image:2p8r.png

Template:STRUCTURE 2p8r

Crystal structure of the C-terminal domain of C. elegans pre-mRNA splicing factor Prp8 carrying R2303K mutant

Template:ABSTRACT PUBMED 17473007

About this Structure

2P8R is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.

Reference

Crystal structure of the C-terminal domain of splicing factor Prp8 carrying retinitis pigmentosa mutants., Zhang L, Shen J, Guarnieri MT, Heroux A, Yang K, Zhao R, Protein Sci. 2007 Jun;16(6):1024-31. Epub 2007 May 1. PMID:17473007

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