This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2p8r

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2p8r.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2p8r.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2p8r| PDB=2p8r | SCENE= }}
{{STRUCTURE_2p8r| PDB=2p8r | SCENE= }}
-
'''Crystal structure of the C-terminal domain of C. elegans pre-mRNA splicing factor Prp8 carrying R2303K mutant'''
+
===Crystal structure of the C-terminal domain of C. elegans pre-mRNA splicing factor Prp8 carrying R2303K mutant===
-
==Overview==
+
<!--
-
Prp8 is a critical pre-mRNA splicing factor. Prp8 is proposed to help form and stabilize the spliceosome catalytic core and to be an important regulator of spliceosome activation. Mutations in human Prp8 (hPrp8) cause a severe form of the genetic disorder retinitis pigmentosa, RP13. Understanding the molecular mechanism of Prp8's function in pre-mRNA splicing and RP13 has been hindered by its large size (over 2000 amino acids) and remarkably low-sequence similarity with other proteins. Here we present the crystal structure of the C-terminal domain (the last 273 residues) of Caenorhabditis elegans Prp8 (cPrp8). The core of the C-terminal domain is an alpha/beta structure that forms the MPN (Mpr1, Pad1 N-terminal) fold but without Zn(2+) coordination. We propose that the C-terminal domain is a protein interaction domain instead of a Zn(2+)-dependent metalloenzyme as proposed for some MPN proteins. Mapping of RP13 mutants on the Prp8 structure suggests that these residues constitute a binding surface between Prp8 and other partner(s), and the disruption of this interaction provides a plausible molecular mechanism for RP13.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17473007}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17473007 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17473007}}
==About this Structure==
==About this Structure==
Line 31: Line 35:
[[Category: Splicing]]
[[Category: Splicing]]
[[Category: Translation]]
[[Category: Translation]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:37:02 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:33:46 2008''

Revision as of 00:33, 28 July 2008

Image:2p8r.png

Template:STRUCTURE 2p8r

Crystal structure of the C-terminal domain of C. elegans pre-mRNA splicing factor Prp8 carrying R2303K mutant

Template:ABSTRACT PUBMED 17473007

About this Structure

2P8R is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.

Reference

Crystal structure of the C-terminal domain of splicing factor Prp8 carrying retinitis pigmentosa mutants., Zhang L, Shen J, Guarnieri MT, Heroux A, Yang K, Zhao R, Protein Sci. 2007 Jun;16(6):1024-31. Epub 2007 May 1. PMID:17473007

Page seeded by OCA on Mon Jul 28 03:33:46 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2p8r"
Personal tools