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1xfq

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[[Image:1xfq.gif|left|200px]]
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{{STRUCTURE_1xfq| PDB=1xfq | SCENE= }}
{{STRUCTURE_1xfq| PDB=1xfq | SCENE= }}
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'''structure of the blue shifted intermediate state of the photoactive yellow protein lacking the N-terminal part'''
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===structure of the blue shifted intermediate state of the photoactive yellow protein lacking the N-terminal part===
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==Overview==
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The N-terminally truncated variant of photoactive yellow protein (Delta25-PYP) undergoes a very similar photocycle as the corresponding wild-type protein (WT-PYP), although the lifetime of its light-illuminated (pB) state is much longer. This has allowed determination of the structure of both its dark- (pG) as well as its pB-state in solution by nuclear magnetic resonance (NMR) spectroscopy. The pG structure shows a well-defined fold, similar to WT-PYP and the X-ray structure of the pG state of Delta25-PYP. In the long-lived photocycle intermediate pB, the central beta sheet is still intact, as well as a small part of one alpha helix. The remainder of pB is unfolded and highly flexible, as evidenced by results from proton-deuterium exchange and NMR relaxation studies. Thus, the partially unfolded nature of the presumed signaling state of PYP in solution, as suggested previously, has now been structurally demonstrated.
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The line below this paragraph, {{ABSTRACT_PUBMED_16004868}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16004868 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16004868}}
==About this Structure==
==About this Structure==
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1XFQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XFQ OCA].
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1XFQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XFQ OCA].
==Reference==
==Reference==
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[[Category: Nuland, N A.van.]]
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[[Category: Pas domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:58:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:56:40 2008''

Revision as of 00:56, 28 July 2008

Image:1xfq.png

Template:STRUCTURE 1xfq

structure of the blue shifted intermediate state of the photoactive yellow protein lacking the N-terminal part

Template:ABSTRACT PUBMED 16004868

About this Structure

1XFQ is a Single protein structure of sequence from Halorhodospira halophila. Full experimental information is available from OCA.

Reference

The solution structure of a transient photoreceptor intermediate: Delta25 photoactive yellow protein., Bernard C, Houben K, Derix NM, Marks D, van der Horst MA, Hellingwerf KJ, Boelens R, Kaptein R, van Nuland NA, Structure. 2005 Jul;13(7):953-62. PMID:16004868

Page seeded by OCA on Mon Jul 28 03:56:40 2008

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