1lb8

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Revision as of 18:19, 20 November 2007

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spinqualitylabelsall models 1lb8, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of the Non-desensitizing GluR2 ligand binding core mutant (S1S2J-L483Y) in complex with AMPA at 2.3 resolution

Overview

Ligand-gated ion channels transduce chemical signals into electrical, impulses by opening a transmembrane pore in response to binding one or, more neurotransmitter molecules. After activation, many ligand-gated ion, channels enter a desensitized state in which the neurotransmitter remains, bound but the ion channel is closed. Although receptor desensitization is, crucial to the functioning of many ligand-gated ion channels in vivo, the, molecular basis of this important process has until now defied analysis., Using the GluR2 AMPA-sensitive glutamate receptor, we show here that the, ligand-binding cores form dimers and that stabilization of the intradimer, interface by either mutations or allosteric modulators reduces, desensitization. Perturbations that destabilize the interface enhance, desensitization. Receptor activation involves conformational changes, within each subunit that result in an increase in the separation of, portions of the receptor that are linked to the ion channel. Our analysis, defines the dimer interface in the resting and activated state, indicates, how ligand binding is coupled to gating, and suggests modes of dimer dimer, interaction in the assembled tetramer. Desensitization occurs through, rearrangement of the dimer interface, which disengages the agonist-induced, conformational change in the ligand-binding core from the ion channel, gate.

About this Structure

1LB8 is a Single protein structure of sequence from Rattus norvegicus with AMQ as ligand. Full crystallographic information is available from OCA.

Reference

Mechanism of glutamate receptor desensitization., Sun Y, Olson R, Horning M, Armstrong N, Mayer M, Gouaux E, Nature. 2002 May 16;417(6886):245-53. PMID:12015593

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