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| - | [[Image:1szk.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1szk| PDB=1szk | SCENE= }} | | {{STRUCTURE_1szk| PDB=1szk | SCENE= }} |
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| - | '''The structure of gamma-aminobutyrate aminotransferase mutant: E211S'''
| + | ===The structure of gamma-aminobutyrate aminotransferase mutant: E211S=== |
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| - | ==Overview==
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| - | The E. coli isozyme of gamma-aminobutyrate aminotransferase (GABA-AT) is a tetrameric pyridoxal phosphate-dependent enzyme that catalyzes transamination between primary amines and alpha-keto acids. The roles of the active site residues V241, E211, and I50 in the GABA-AT mechanism have been probed by site-directed mutagenesis. The beta-branched side chain of V241 facilitates formation of external aldimine intermediates with primary amine substrates, while E211 provides charge compensation of R398 selectively in the primary amine half-reaction and I50 forms a hydrophobic lid at the top of the substrate binding site. The structures of the I50Q, V241A, and E211S mutants were solved by X-ray crystallography to resolutions of 2.1, 2.5, and 2.52 A, respectively. The structure of GABA-AT is similar in overall fold and active site structure to that of dialkylglycine decarboxylase, which catalyzes both transamination and decarboxylation half-reactions in its normal catalytic cycle. Therefore, an attempt was made to convert GABA-AT into a decarboxylation-dependent aminotransferase similar to dialkylglycine decarboxylase by systematic mutation of E. coli GABA-AT active site residues. Two of the twelve mutants presented, E211S/I50G/C77K and E211S/I50H/V80D, have approximately 10-fold higher decarboxylation activities than the wild-type enzyme, and the E211S/I50H/V80D has formally changed the reaction specificity to that of a decarboxylase.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_15723541}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15723541 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15723541}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Toney, M D.]] | | [[Category: Toney, M D.]] |
| | [[Category: Gaba-at]] | | [[Category: Gaba-at]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:18:58 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:00:14 2008'' |
Revision as of 01:00, 28 July 2008
Template:STRUCTURE 1szk
The structure of gamma-aminobutyrate aminotransferase mutant: E211S
Template:ABSTRACT PUBMED 15723541
About this Structure
1SZK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Kinetic and crystallographic analysis of active site mutants of Escherichia coli gamma-aminobutyrate aminotransferase., Liu W, Peterson PE, Langston JA, Jin X, Zhou X, Fisher AJ, Toney MD, Biochemistry. 2005 Mar 1;44(8):2982-92. PMID:15723541
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