2am3

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{{STRUCTURE_2am3| PDB=2am3 | SCENE= }}
{{STRUCTURE_2am3| PDB=2am3 | SCENE= }}
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'''Crystal Structure of N-Acetylglucosaminyltransferase I in Complex with UDP-Glucose'''
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===Crystal Structure of N-Acetylglucosaminyltransferase I in Complex with UDP-Glucose===
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==Overview==
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The Golgi-resident glycosyltransferase, UDP-N-acetyl-d-glucosamine:alpha-3-d-mannoside beta-1,2-N-acetylglucosaminyltransferase I (GnT I), initiates the conversion of high-mannose oligosaccharides to complex and hybrid structures in the biosynthesis of N-linked glycans. Reported here are the X-ray crystal structures of GnT I in complex with UDP-CH2-GlcNAc (a non-hydrolyzable C-glycosidic phosphonate), UDP-2-deoxy-2-fluoro-glucose, UDP-glucose and UDP. Collectively, these structures provide evidence for the importance of the GlcNAc moiety and its N-acetyl group in donor substrate binding, as well as insight into the role played by the flexible 318-330 loop in substrate binding and product release. In addition, the UDP-CH2-GlcNAc complex reveals a well-defined glycerol molecule poised for nucleophilic attack on the C1 atom of the donor substrate analogue. The position and orientation of this glycerol molecule have allowed us to model the binding of the Manalpha1,3Manbeta1 moiety of the acceptor substrate and, based on the model, to suggest a rationalization for the main determinants of GnT I acceptor specificity.
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{{ABSTRACT_PUBMED_16769084}}
==About this Structure==
==About this Structure==
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[[Category: Udp-glcnac]]
[[Category: Udp-glcnac]]
[[Category: Udp-glucose]]
[[Category: Udp-glucose]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:07:05 2008''

Revision as of 01:07, 28 July 2008

Image:2am3.png

Template:STRUCTURE 2am3

Crystal Structure of N-Acetylglucosaminyltransferase I in Complex with UDP-Glucose

Template:ABSTRACT PUBMED 16769084

About this Structure

2AM3 is a Single protein structure of sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA.

Reference

X-ray crystal structures of rabbit N-acetylglucosaminyltransferase I (GnT I) in complex with donor substrate analogues., Gordon RD, Sivarajah P, Satkunarajah M, Ma D, Tarling CA, Vizitiu D, Withers SG, Rini JM, J Mol Biol. 2006 Jun 30;360(1):67-79. Epub 2006 May 12. PMID:16769084

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