1qmy

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{{STRUCTURE_1qmy| PDB=1qmy | SCENE= }}
{{STRUCTURE_1qmy| PDB=1qmy | SCENE= }}
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'''FMDV LEADER PROTEASE (LBSHORT-C51A-C133S)'''
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===FMDV LEADER PROTEASE (LBSHORT-C51A-C133S)===
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==Overview==
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The structures of the two leader protease (Lpro) variants of foot-and-mouth disease virus known to date were solved using crystals in which molecules were organized as molecular fibers. Such crystals diffract to a resolution of only approximately 3 A. This singular, pseudo-polymeric organization is present in a new Lpro crystal form showing a cubic packing. As molecular fiber formation appeared unrelated to crystallization conditions, we mutated the reactive cysteine 133 residue, which makes a disulfide bridge between adjacent monomers in the fibers, to serine. None of the intermolecular contacts found in the molecular fibers was present in crystals of this variant. Analysis of this Lpro structure, refined at 1.9 A resolution, enables a detailed definition of the active center of the enzyme, including the solvent organization. Assay of Lpro activity on a fluorescent hexapeptide substrate showed that Lpro, in contrast to papain, was highly sensitive to increases in the cation concentration and was active only across a narrow pH range. Examination of the Lpro structure revealed that three aspartate residues near the active site, not present in papain-like enzymes, are probably responsible for these properties.
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(as it appears on PubMed at http://www.pubmed.gov), where 11183785 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11183785}}
==About this Structure==
==About this Structure==
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[[Category: Picornaviral proteinase]]
[[Category: Picornaviral proteinase]]
[[Category: Sulfhydryl proteinase]]
[[Category: Sulfhydryl proteinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:28:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:13:47 2008''

Revision as of 01:13, 28 July 2008

Image:1qmy.png

Template:STRUCTURE 1qmy

FMDV LEADER PROTEASE (LBSHORT-C51A-C133S)

Template:ABSTRACT PUBMED 11183785

About this Structure

1QMY is a Single protein structure of sequence from Foot-and-mouth disease virus. Full crystallographic information is available from OCA.

Reference

Structural and biochemical features distinguish the foot-and-mouth disease virus leader proteinase from other papain-like enzymes., Guarne A, Hampoelz B, Glaser W, Carpena X, Tormo J, Fita I, Skern T, J Mol Biol. 2000 Oct 6;302(5):1227-40. PMID:11183785

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