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2bjw

From Proteopedia

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{{STRUCTURE_2bjw| PDB=2bjw | SCENE= }}
{{STRUCTURE_2bjw| PDB=2bjw | SCENE= }}
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'''PSPF AAA DOMAIN'''
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===PSPF AAA DOMAIN===
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==Overview==
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Activators of bacterial sigma54-RNA polymerase holoenzyme are mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis to activate transcription. We have determined by cryogenic electron microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition state analog in complex with its basal factor, sigma54. By fitting the crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we identified two loops involved in binding sigma54. Comparing enhancer-binding structures in different nucleotide states and mutational analysis led us to propose nucleotide-dependent conformational changes that free the loops for association with sigma54.
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(as it appears on PubMed at http://www.pubmed.gov), where 15790859 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15790859}}
==About this Structure==
==About this Structure==
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[[Category: Sigma54 activator]]
[[Category: Sigma54 activator]]
[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:23:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:14:26 2008''

Revision as of 01:14, 28 July 2008

Image:2bjw.png

Template:STRUCTURE 2bjw

PSPF AAA DOMAIN

Template:ABSTRACT PUBMED 15790859

About this Structure

2BJW is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural insights into the activity of enhancer-binding proteins., Rappas M, Schumacher J, Beuron F, Niwa H, Bordes P, Wigneshweraraj S, Keetch CA, Robinson CV, Buck M, Zhang X, Science. 2005 Mar 25;307(5717):1972-5. PMID:15790859

Page seeded by OCA on Mon Jul 28 04:14:26 2008

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