1lci
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(New page: 200px<br /><applet load="1lci" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lci, resolution 2.0Å" /> '''FIREFLY LUCIFERASE'''...)
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Revision as of 18:21, 20 November 2007
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FIREFLY LUCIFERASE
Overview
BACKGROUND. Firefly luciferase is a 62 kDa protein that catalyzes the, production of light. In the presence of MgATP and molecular oxygen, the, enzyme oxidizes its substrate, firefly luciferin, emitting yellow-green, light. The reaction proceeds through activation of the substrate to form, an adenylate intermediate. Firefly luciferase shows extensive sequence, homology with a number of enzymes that utilize ATP in adenylation, reactions. RESULTS. We have determined the crystal structure of firefly, luciferase at 2.0 A resolution. The protein is folded into two compact, domains. The large N-terminal domain consists of a beta-barrel and two, beta-sheets. The sheets are flanked by alpha-helices to form an, alphabetaalphabetaalpha five-layered structure. The C-terminal portion of, the molecule forms a distinct domain, which is separated from the, N-terminal domain by a wide cleft. CONCLUSIONS. Firefly luciferase is the, first member of a superfamily of homologous enzymes, which includes, acyl-coenzyme A ligases and peptide synthetases, to have its structure, characterized. The residues conserved within the superfamily are located, on the surfaces of the two domains on either side of the cleft, but are, too far apart to interact simultaneously with the substrates. This, suggests that the two domains will close in the course of the reaction., Firefly luciferase has a novel structural framework for catalyzing, adenylate-forming reactions.
About this Structure
1LCI is a Single protein structure of sequence from Photinus pyralis. Active as Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing), with EC number 1.13.12.7 Full crystallographic information is available from OCA.
Reference
Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes., Conti E, Franks NP, Brick P, Structure. 1996 Mar 15;4(3):287-98. PMID:8805533
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