We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

1n1k

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1n1k.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1n1k.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1n1k| PDB=1n1k | SCENE= }}
{{STRUCTURE_1n1k| PDB=1n1k | SCENE= }}
-
'''NMR Structure for d(CCGCGG)2'''
+
===NMR Structure for d(CCGCGG)2===
-
==Overview==
+
<!--
-
Long repeated stretches of d(CCG) and tri-nucleotide are crucial mutations that cause hereditary forms of mental retardation (fragile X-syndrome). Moreover, the alternating (CG) di-nucleotide is one of the candidates for Z-DNA conformation. Solution NMR structure of d(CCGCGG)(2) has been solved and is discussed. The determined NMR solution structure is a distorted highly bent B-DNA conformation with increased flexibility in both terminal residues. This conformation differs significantly from the Z-DNA tetramer structure reported for the same hexamer in the crystal state at similar ionic strength by Malinina and co-workers. Crystal structure of d(CCGCGG)(2) at high salt concentration includes a central alternating tetramer in Z-DNA conformation, while the initial cytosine swings out and forms a Watson-Crick base-pair with the terminal guanine of a symmetry-related molecule. In solution, NMR data for sugar ring puckering combined with restrained molecular dynamics simulations starting from a Z-DNA form show that terminal furanose residues could adopt the conformation required for aromatic bases swinging out. Therefore, tetramer formation could be considered possible once the hexanucleotide had previously adopted the Z-DNA form. This work gives some insight into correlations between anomalous crystal structures and their accessibility in the solution state.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12646170}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12646170 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12646170}}
==About this Structure==
==About this Structure==
-
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N1K OCA].
+
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N1K OCA].
==Reference==
==Reference==
Line 25: Line 29:
[[Category: Nmr]]
[[Category: Nmr]]
[[Category: Recombination-like]]
[[Category: Recombination-like]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:58:34 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:17:41 2008''

Revision as of 01:17, 28 July 2008

Image:1n1k.png

Template:STRUCTURE 1n1k

NMR Structure for d(CCGCGG)2

Template:ABSTRACT PUBMED 12646170

About this Structure

Full experimental information is available from OCA.

Reference

NMR study of hexanucleotide d(CCGCGG)2 containing two triplet repeats of fragile X syndrome., Monleon D, Esteve V, Celda B, Biochem Biophys Res Commun. 2003 Mar 28;303(1):81-90. PMID:12646170

Page seeded by OCA on Mon Jul 28 04:17:41 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1n1k"
Personal tools