We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

2ghy

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2ghy.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2ghy.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2ghy| PDB=2ghy | SCENE= }}
{{STRUCTURE_2ghy| PDB=2ghy | SCENE= }}
-
'''Novel Crystal Form of the ColE1 Rom Protein'''
+
===Novel Crystal Form of the ColE1 Rom Protein===
-
==Overview==
+
<!--
-
The RNA I modulator protein (Rom) acts as a co-regulator of ColE1 plasmid copy number by binding to RNA kissing hairpins and stabilizing their interaction. The structure of Rom has been determined in a new crystal form from X-ray diffraction data to 2.5 A resolution. In this structure, a dimer of the 57-amino-acid protein is found in the asymmetric unit. Each subunit consists almost entirely of two antiparallel alpha-helices joined by a short hairpin bend. The dimer contains a non-crystallographic twofold axis and forms a highly regular four-alpha-helical bundle. The structural packing in this novel crystal form is different from previously known Rom structures. The asymmetric unit contains one dimer, giving a crystal volume per protein weight (V(M)) of 1.83 A(3) Da(-1) and a low solvent content of 30%. Strong packing interactions and low solvation are characteristic of the structure. The Rom protein was cocrystallized with the Tar-Tar* kissing hairpin RNA. Although the electron-density maps do not show bound RNA, altered conformations in the side chains of Rom that are known to be involved in RNA binding have been identified. These results provide additional information about Rom protein conformational flexibility and suggest that the presence of a highly charged polymer such as RNA can promote tight packing of an RNA-binding protein, even when the RNA itself is not observed in the crystal.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16699189}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16699189 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16699189}}
==About this Structure==
==About this Structure==
Line 32: Line 36:
[[Category: Rna one modulator protein]]
[[Category: Rna one modulator protein]]
[[Category: Structural packing]]
[[Category: Structural packing]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:07:46 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:22:16 2008''

Revision as of 01:22, 28 July 2008

Image:2ghy.png

Template:STRUCTURE 2ghy

Novel Crystal Form of the ColE1 Rom Protein

Template:ABSTRACT PUBMED 16699189

About this Structure

2GHY is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Novel crystal form of the ColE1 Rom protein., Jang SB, Jeong MS, Carter RJ, Holbrook EL, Comolli LR, Holbrook SR, Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):619-27. Epub 2006, May 12. PMID:16699189

Page seeded by OCA on Mon Jul 28 04:22:16 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ghy"
Personal tools