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| - | [[Image:1nd0.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1nd0| PDB=1nd0 | SCENE= }} | | {{STRUCTURE_1nd0| PDB=1nd0 | SCENE= }} |
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| - | '''CATIONIC CYCLIZATION ANTIBODY 4C6 COMPLEX WITH TRANSITION STATE ANALOG'''
| + | ===CATIONIC CYCLIZATION ANTIBODY 4C6 COMPLEX WITH TRANSITION STATE ANALOG=== |
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| - | ==Overview==
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| - | Antibody 4C6 efficiently catalyzes a cationic cyclization reaction. Crystal structures of the antibody 4C6 Fab in complex with benzoic acid and in complex with its eliciting hapten were determined to 1.30A and 2.45A resolution, respectively. These crystal structures, together with computational analysis, have elucidated a possible mechanism for the monocyclization reaction. The hapten complex revealed a combining site pocket with high shape complementarity to the hapten. This active site cleft is dominated by aromatic residues that shield the highly reactive carbocation intermediates from solvent and stabilize the carbocation intermediates through cation-pi interactions. Modeling of an acyclic olefinic sulfonate ester substrate and the transition state (TS) structures shows that the chair-like transition state is favored, and trapping by water directly produces trans-2-(dimethylphenylsilyl)-cyclohexanol, whereas the less favored boat-like transition state leads to cyclohexene. The only significant change observed upon hapten binding is a side-chain rotation of Trp(L89), which reorients to form the base of the combining site. Intriguingly, a benzoic acid molecule was sequestered in the combining site of the unliganded antibody. The 4C6 active site was compared to that observed in a previously reported tandem cyclization antibody 19A4 hapten complex. These cationic cyclization antibodies exhibit convergent structural features with terpenoid cyclases that appear to be important for catalysis.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_12742019}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 12742019 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_12742019}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Cationic cyclization reaction]] | | [[Category: Cationic cyclization reaction]] |
| | [[Category: Immunoglobulin]] | | [[Category: Immunoglobulin]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:23:13 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:24:29 2008'' |
Revision as of 01:24, 28 July 2008
Template:STRUCTURE 1nd0
CATIONIC CYCLIZATION ANTIBODY 4C6 COMPLEX WITH TRANSITION STATE ANALOG
Template:ABSTRACT PUBMED 12742019
About this Structure
1ND0 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structural basis for antibody catalysis of a cationic cyclization reaction., Zhu X, Heine A, Monnat F, Houk KN, Janda KD, Wilson IA, J Mol Biol. 2003 May 23;329(1):69-83. PMID:12742019
Page seeded by OCA on Mon Jul 28 04:24:29 2008
