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2ork

From Proteopedia

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{{STRUCTURE_2ork| PDB=2ork | SCENE= }}
{{STRUCTURE_2ork| PDB=2ork | SCENE= }}
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'''crystal structure of the trimeric neck and carbohydrate recognition domain of human surfactant protein D in complex with inositol-1-phosphate'''
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===crystal structure of the trimeric neck and carbohydrate recognition domain of human surfactant protein D in complex with inositol-1-phosphate===
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==Overview==
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Surfactant protein D (SP-D) plays important roles in lung host defense. However, it can also recognize specific host molecules and contributes to surfactant homeostasis. The major known surfactant-associated ligand is phosphatidylinositol (PI). Trimeric neck-carbohydrate recognition domains (NCRDs) of rat and human SP-D exhibited dose-dependent, calcium-dependent, and inositol-sensitive binding to solid-phase PI and to multilamellar PI liposomes. However, the rat protein exhibited a &gt;5-fold higher affinity for solid-phase PI than the human NCRD. In addition, human dodecamers, but not full-length human trimers, efficiently coprecipitated with multilamellar PI liposomes in the presence of calcium. A human NCRD mutant resembling the rat and mouse proteins at position 343 (hR343K) showed much stronger binding to PI. A reciprocal rat mutant with arginine at the position of lysine 343 (rK343R) showed weak binding to PI, even weaker than that of the wild-type human protein. Crystal complexes of the human trimeric NCRD with myoinositol and inositol 1-phosphate showed binding of the equatorial OH groups of the cyclitol ring of the inositol to calcium at the carbohydrate binding site. Myoinositol binding occurred in two major orientations, while inositol 1-phosphate appeared primarily constrained to a single, different orientation. Our studies directly implicate the CRD in PI binding and reveal unexpected species differences in PI recognition that can be largely attributed to the side chain of residue 343. In addition, the studies indicate that oligomerization of trimeric subunits is an important determinant of recognition of PI by human SP-D.
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{{ABSTRACT_PUBMED_17417879}}
==About this Structure==
==About this Structure==
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[[Category: Surfactant protein]]
[[Category: Surfactant protein]]
[[Category: Trimeric]]
[[Category: Trimeric]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:31:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:28:31 2008''

Revision as of 01:28, 28 July 2008

Image:2ork.png

Template:STRUCTURE 2ork

crystal structure of the trimeric neck and carbohydrate recognition domain of human surfactant protein D in complex with inositol-1-phosphate

Template:ABSTRACT PUBMED 17417879

About this Structure

2ORK is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Critical role of Arg/Lys343 in the species-dependent recognition of phosphatidylinositol by pulmonary surfactant protein D., Crouch E, McDonald B, Smith K, Roberts M, Mealy T, Seaton B, Head J, Biochemistry. 2007 May 1;46(17):5160-9. Epub 2007 Apr 7. PMID:17417879

Page seeded by OCA on Mon Jul 28 04:28:31 2008

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