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| - | [[Image:2a97.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2a97| PDB=2a97 | SCENE= }} | | {{STRUCTURE_2a97| PDB=2a97 | SCENE= }} |
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| - | '''Crystal structure of catalytic domain of Clostridium botulinum neurotoxin serotype F'''
| + | ===Crystal structure of catalytic domain of Clostridium botulinum neurotoxin serotype F=== |
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| - | ==Overview==
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| - | The seven serologically distinct Clostridium botulinum neurotoxins (BoNTs A-G) are zinc endopeptidases which block the neurotransmitter release by cleaving one of the three proteins of the soluble N-ethylmaleimide-sensitive-factor attachment protein receptor complex (SNARE complex) essential for the fusion of vesicles containing neurotransmitters with target membranes. These metallopeptidases exhibit unique specificity for the substrates and peptide bonds they cleave. Development of countermeasures and therapeutics for BoNTs is a priority because of their extreme toxicity and potential misuse as biowarfare agents. Though they share sequence homology and structural similarity, the structural information on each one of them is required to understand the mechanism of action of all of them because of their specificity. Unraveling the mechanism will help in the ultimate goal of developing inhibitors as antibotulinum drugs for the toxins. Here, we report the high-resolution structure of active BoNT/F catalytic domain in two crystal forms. The structure was exploited for modeling the substrate binding and identifying the S1' subsite and the putative exosites which are different from BoNT/A or BoNT/B. The orientation of docking of the substrate at the active site is consistent with the experimental BoNT/A-LC:SNAP-25 peptide model and our proposed model for BoNT/E-LC:SNAP-25. | + | The line below this paragraph, {{ABSTRACT_PUBMED_16128577}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16128577 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16128577}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: X-ray]] | | [[Category: X-ray]] |
| | [[Category: Zinc endopeptidase]] | | [[Category: Zinc endopeptidase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:46:03 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:29:47 2008'' |
Revision as of 01:29, 28 July 2008
Template:STRUCTURE 2a97
Crystal structure of catalytic domain of Clostridium botulinum neurotoxin serotype F
Template:ABSTRACT PUBMED 16128577
About this Structure
2A97 is a Single protein structure of sequence from Clostridium botulinum. Full crystallographic information is available from OCA.
Reference
Structural analysis of botulinum neurotoxin serotype F light chain: implications on substrate binding and inhibitor design., Agarwal R, Binz T, Swaminathan S, Biochemistry. 2005 Sep 6;44(35):11758-65. PMID:16128577
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