1rtt

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{{STRUCTURE_1rtt| PDB=1rtt | SCENE= }}
{{STRUCTURE_1rtt| PDB=1rtt | SCENE= }}
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'''Crystal structure determination of a putative NADH-dependent reductase using sulfur anomalous signal'''
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===Crystal structure determination of a putative NADH-dependent reductase using sulfur anomalous signal===
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==Overview==
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The availability of high-intensity synchrotron facilities, technological advances in data-collection techniques and improved data-reduction and crystallographic software have ushered in a new era in high-throughput macromolecular crystallography. Here, the de novo automated crystal structure determination at 1.28 A resolution of an NAD(P)H-dependent FMN reductase flavoprotein from Pseudomonas aeruginosa PA01-derived protein Q9I4D4 using the anomalous signal from an unusually small number of S atoms is reported. Although this protein lacks the flavodoxin key fingerprint motif [(T/S)XTGXT], it has been confirmed to bind flavin mononucleotide and the binding site was identified via X-ray crystallography. This protein contains a novel flavin mononucleotide-binding site GSLRSGSYN, which has not been previously reported. Detailed statistics pertaining to sulfur phasing and other factors contributing to structure determination are discussed. Structural comparisons of the apoenzyme and the protein complexed with flavin mononucleotide show conformational changes on cofactor binding. NADPH-dependent activity has been confirmed with biochemical assays.
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(as it appears on PubMed at http://www.pubmed.gov), where 16552139 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16552139}}
==About this Structure==
==About this Structure==
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[[Category: Sad with sulfur]]
[[Category: Sad with sulfur]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:53:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:34:16 2008''

Revision as of 01:34, 28 July 2008

Image:1rtt.png

Template:STRUCTURE 1rtt

Crystal structure determination of a putative NADH-dependent reductase using sulfur anomalous signal

Template:ABSTRACT PUBMED 16552139

About this Structure

1RTT is a Single protein structure of sequence from Pseudomonas aeruginosa pao1. Full crystallographic information is available from OCA.

Reference

Structure determination of an FMN reductase from Pseudomonas aeruginosa PA01 using sulfur anomalous signal., Agarwal R, Bonanno JB, Burley SK, Swaminathan S, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):383-91. Epub 2006, Mar 18. PMID:16552139

Page seeded by OCA on Mon Jul 28 04:34:16 2008

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