2jwm

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[[Image:2jwm.jpg|left|200px]]
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{{Seed}}
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[[Image:2jwm.png|left|200px]]
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{{STRUCTURE_2jwm| PDB=2jwm | SCENE= }}
{{STRUCTURE_2jwm| PDB=2jwm | SCENE= }}
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'''NMR spatial srtucture of ternary complex kalata B7/Mn2+/DPC micelle'''
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===NMR spatial srtucture of ternary complex kalata B7/Mn2+/DPC micelle===
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==Overview==
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The cyclotides are the family of hydrophobic bioactive plant peptides, characterized by a circular protein backbone and three knot forming disulfide bonds. It is believed that membrane activity of the cyclotides underlines their antimicrobial, cytotoxic and hemolytic properties, but the specific interactions with divalent cations can be also involved. To assess the mode of membrane interaction and divalent cation coordination in cyclotides, the spatial structure of the Mobius cyclotide Kalata B7 from the African perennial plant Oldenlandia affinis was determined in the presence of anisotropic membrane mimetic (dodecylphosphocholine micelles). The model of peptide/cation/micelle complex was built using 5-doxylstearate and Mn(2+) relaxation probes. Results show that the peptide binds to the micelle surface with relatively high affinity by two hydrophobic loops (loop 2 - Thr(6)-Leu(7) and loop 5 - Trp(19)-Ile(21)). The partially hydrated divalent cation is coordinated by charged side-chain of Glu(3), aromatic side chain of Tyr(11) and free carbonyls of Thr(4) and Thr(9), and is located in direct contact with the polar head-groups of detergent. The comparison with data about other cyclotides indicates that divalent cation coordination is the invariant property of all cyclotides, but the mode of peptide/membrane interactions is varied. Probably, the specific cation/peptide interactions play a major, but yet not known, role in the biological activity of the cyclotides.
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{{ABSTRACT_PUBMED_18295894}}
==About this Structure==
==About this Structure==
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2JWM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oldenlandia_affinis Oldenlandia affinis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JWM OCA].
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2JWM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oldenlandia_affinis Oldenlandia affinis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JWM OCA].
==Reference==
==Reference==
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[[Category: Plant defense]]
[[Category: Plant defense]]
[[Category: Plant protein]]
[[Category: Plant protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:21:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:37:54 2008''

Revision as of 01:37, 28 July 2008

Image:2jwm.png

Template:STRUCTURE 2jwm

NMR spatial srtucture of ternary complex kalata B7/Mn2+/DPC micelle

Template:ABSTRACT PUBMED 18295894

About this Structure

2JWM is a Single protein structure of sequence from Oldenlandia affinis. Full experimental information is available from OCA.

Reference

Divalent cation coordination and mode of membrane interaction in cyclotides: NMR spatial structure of ternary complex Kalata B7/Mn(2+)/DPC micelle., Shenkarev ZO, Nadezhdin KD, Lyukmanova EN, Sobol VA, Skjeldal L, Arseniev AS, J Inorg Biochem. 2008 Jan 21;. PMID:18295894

Page seeded by OCA on Mon Jul 28 04:37:54 2008

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