1rc8

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{{STRUCTURE_1rc8| PDB=1rc8 | SCENE= }}
{{STRUCTURE_1rc8| PDB=1rc8 | SCENE= }}
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'''T4 Polynucleotide Kinase bound to 5'-GTCAC-3' ssDNA'''
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===T4 Polynucleotide Kinase bound to 5'-GTCAC-3' ssDNA===
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==Overview==
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T4 phage polynucleotide kinase (PNK) displays 5'-hydroxyl kinase, 3'-phosphatase and 2',3'-cyclic phosphodiesterase activities. The enzyme phosphorylates the 5' hydroxyl termini of a wide variety of nucleic acid substrates, a behavior studied here through the determination of a series of crystal structures with single-stranded (ss)DNA oligonucleotide substrates of various lengths and sequences. In these structures, the 5' ribose hydroxyl is buried in the kinase active site in proper alignment for phosphoryl transfer. Depending on the ssDNA length, the first two or three nucleotide bases are well ordered. Numerous contacts are made both to the phosphoribosyl backbone and to the ordered bases. The position, side chain contacts and internucleotide stacking interactions of the ordered bases are strikingly different for a 5'-GT DNA end than for a 5'-TG end. The base preferences displayed at those positions by PNK are attributable to differences in the enzyme binding interactions and in the DNA conformation for each unique substrate molecule.
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(as it appears on PubMed at http://www.pubmed.gov), where 14754987 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14754987}}
==About this Structure==
==About this Structure==
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[[Category: Phosphatase]]
[[Category: Phosphatase]]
[[Category: Ssdna]]
[[Category: Ssdna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:19:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:42:25 2008''

Revision as of 01:42, 28 July 2008

Image:1rc8.png

Template:STRUCTURE 1rc8

T4 Polynucleotide Kinase bound to 5'-GTCAC-3' ssDNA

Template:ABSTRACT PUBMED 14754987

About this Structure

1RC8 is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

Recognition of DNA substrates by T4 bacteriophage polynucleotide kinase., Eastberg JH, Pelletier J, Stoddard BL, Nucleic Acids Res. 2004 Jan 30;32(2):653-60. Print 2004. PMID:14754987

Page seeded by OCA on Mon Jul 28 04:42:25 2008

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