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| | {{STRUCTURE_1wm2| PDB=1wm2 | SCENE= }} | | {{STRUCTURE_1wm2| PDB=1wm2 | SCENE= }} |
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| - | '''Crystal structure of human SUMO-2 protein'''
| + | ===Crystal structure of human SUMO-2 protein=== |
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| - | ==Overview==
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| - | The SUMO proteins are a class of small ubiquitin-like modifiers. SUMO is attached to a specific lysine side chain on the target protein via an isopeptide bond with its C-terminal glycine. There are at least four SUMO proteins in humans, which are involved in protein trafficking and targeting. A truncated human SUMO-2 protein that contains residues 9-93 was expressed in Escherichia coli and crystallized in two different unit cells, with dimensions of a=b=75.25 A, c=29.17 A and a=b=74.96 A, c=33.23 A, both belonging to the rhombohedral space group R3. They diffracted X-rays to 1.6 A and 1.2 A resolution, respectively. The structures were determined by molecular replacement using the yeast SMT3 protein as a search model. Subsequent refinements yielded R/Rfree values of 0.169/0.190 and 0.119/0.185, at 1.6 A and 1.2 A, respectively. The peptide folding of SUMO-2 consists of a half-open beta-barrel and two flanking alpha-helices with secondary structural elements arranged as betabetaalphabetabetaalphabeta in the sequence, identical to those of ubiquitin, SMT3 and SUMO-1. Comparison of SUMO-2 with SUMO-1 showed a surface region near the C terminus with significantly different charge distributions. This may explain their distinct intracellular locations. In addition, crystal-packing analysis suggests a possible trimeric assembly of the SUMO-2 protein, of which the biological significance remains to be determined. | + | The line below this paragraph, {{ABSTRACT_PUBMED_15479240}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15479240 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15479240}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Two helice]] | | [[Category: Two helice]] |
| | [[Category: Ubiquitin fold]] | | [[Category: Ubiquitin fold]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:51:40 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:42:59 2008'' |
Revision as of 01:43, 28 July 2008
Template:STRUCTURE 1wm2
Crystal structure of human SUMO-2 protein
Template:ABSTRACT PUBMED 15479240
About this Structure
1WM2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structures of the human SUMO-2 protein at 1.6 A and 1.2 A resolution: implication on the functional differences of SUMO proteins., Huang WC, Ko TP, Li SS, Wang AH, Eur J Biochem. 2004 Oct;271(20):4114-22. PMID:15479240
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