1lg7
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(New page: 200px<br /><applet load="1lg7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lg7, resolution 1.96Å" /> '''Crystal structure of...)
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Revision as of 18:25, 20 November 2007
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Crystal structure of Vesicular Stomatitis Virus Matrix Protein
Overview
The vesicular stomatitis virus (VSV) matrix protein (M) interacts with, cellular membranes, self-associates and plays a major role in virus, assembly and budding. We present the crystallographic structure, determined at 1.96 A resolution, of a soluble thermolysin resistant core, of VSV M. The fold is a new fold shared by the other vesiculovirus matrix, proteins. The structure accounts for the loss of stability of M, temperature-sensitive mutants deficient in budding, and reveals a flexible, loop protruding from the globular core that is important for, self-assembly. Membrane floatation shows that, together with the M, lysine-rich N-terminal peptide, a second domain of the protein is involved, in membrane binding. Indeed, the structure reveals a hydrophobic surface, located close to the hydrophobic loop and surrounded by conserved basic, residues that may constitute this domain. Lastly, comparison of the, negative-stranded virus matrix proteins with retrovirus Gag proteins, suggests that the flexible link between their major membrane binding, domain and the rest of the structure is a common feature shared by these, proteins involved in budding and virus assembly.
About this Structure
1LG7 is a Single protein structure of sequence from Vesicular stomatitis virus. Full crystallographic information is available from OCA.
Reference
Crystal structure of vesicular stomatitis virus matrix protein., Gaudier M, Gaudin Y, Knossow M, EMBO J. 2002 Jun 17;21(12):2886-92. PMID:12065402
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