From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| Line 1: |
Line 1: |
| - | [[Image:2jyp.jpg|left|200px]] | + | {{Seed}} |
| | + | [[Image:2jyp.png|left|200px]] |
| | | | |
| | <!-- | | <!-- |
| Line 9: |
Line 10: |
| | {{STRUCTURE_2jyp| PDB=2jyp | SCENE= }} | | {{STRUCTURE_2jyp| PDB=2jyp | SCENE= }} |
| | | | |
| - | '''Coordinates for lowest energy structure of Aragonite protein-7, C-terminal domain'''
| + | ===Coordinates for lowest energy structure of Aragonite protein-7, C-terminal domain=== |
| | | | |
| | | | |
| - | ==Overview==
| + | <!-- |
| - | The RING or Really Interesting New Gene represents a family of eukaryotic sequences that bind Zn (II) ions and participate in intracellular processes involving protein-protein interaction. Although found in over 400 different proteins, very little is known regarding the structure-function properties of these domains because of the aggregation problems associated with RING sequences. To augment this data set, we report an unusual 36 AA C-terminal sequence of an extracellular matrix mollusk shell protein, AP7, that exhibits partial homology to the RING family. This Cys, His-containing sequence, termed AP7C, binds Zn (II) and other multivalent ions, but does not utilize a tetracoordinate complexation scheme for binding such as that found in Zn (II) finger polypeptides. Moreover, unlike Zn (II) finger and RING domains, this 36 AA can fold into a relatively stable structure in the absence of Zn (II). This folded structure consists of three short helical segments (A, B, and C), with segments A and B separated by a 4 AA type I beta-turn region and segments B and C separated by a 7 AA loop-like region. Interestingly, the putative RING-like region, -RRPFHECALCYSI-, experiences slow conformational exchange between two structural states in solution, most likely in response to imido ring interconversion at P8 and P21. Poisson-Boltzmann solvation calculations reveal that the AP7C molecular surface possesses a cationic region near its N-terminus, which lies adjacent to the 30 AA mineral modification domain in the AP7 protein. Given that the AP7C sequence does not influence mineralization, it is probable that this cationic pseudo-RING region is utilized by the AP7 protein for other tasks such as protein-protein interaction within the mollusk shell matrix. | + | The line below this paragraph, {{ABSTRACT_PUBMED_18298090}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 18298090 is the PubMed ID number. |
| | + | --> |
| | + | {{ABSTRACT_PUBMED_18298090}} |
| | | | |
| | ==About this Structure== | | ==About this Structure== |
| - | 2JYP is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JYP OCA]. | + | 2JYP is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JYP OCA]. |
| | | | |
| | ==Reference== | | ==Reference== |
| Line 26: |
Line 30: |
| | [[Category: Protein c-terminal fragment]] | | [[Category: Protein c-terminal fragment]] |
| | [[Category: Unknown function]] | | [[Category: Unknown function]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 09:24:35 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:43:30 2008'' |
Revision as of 01:43, 28 July 2008
Template:STRUCTURE 2jyp
Coordinates for lowest energy structure of Aragonite protein-7, C-terminal domain
Template:ABSTRACT PUBMED 18298090
About this Structure
2JYP is a Single protein structure. Full experimental information is available from OCA.
Reference
Identification and Structural Characterization of an Unusual RING-Like Sequence within an Extracellular Biomineralization Protein, AP7., Collino S, Kim IW, Evans JS, Biochemistry. 2008 Mar 25;47(12):3745-55. Epub 2008 Feb 26. PMID:18298090
Page seeded by OCA on Mon Jul 28 04:43:30 2008
