2hen

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2hen.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2hen.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2hen| PDB=2hen | SCENE= }}
{{STRUCTURE_2hen| PDB=2hen | SCENE= }}
-
'''Crystal Structure of the EphB2 Receptor Kinase domain in complex with ADP'''
+
===Crystal Structure of the EphB2 Receptor Kinase domain in complex with ADP===
-
==Overview==
+
<!--
-
Eph receptor tyrosine kinases (RTKs) mediate numerous developmental processes. Their activity is regulated by auto-phosphorylation on two tyrosines within the juxtamembrane segment (JMS) immediately N-terminal to the kinase domain (KD). Here, we probe the molecular details of Eph kinase activation through mutational analysis, X-ray crystallography and NMR spectroscopy on auto-inhibited and active EphB2 and EphA4 fragments. We show that a Tyr750Ala gain-of-function mutation in the KD and JMS phosphorylation independently induce disorder of the JMS and its dissociation from the KD. Our X-ray analyses demonstrate that this occurs without major conformational changes to the KD and with only partial ordering of the KD activation segment. However, conformational exchange for helix alphaC in the N-terminal KD lobe and for the activation segment, coupled with increased inter-lobe dynamics, is observed upon kinase activation in our NMR analyses. Overall, our results suggest that a change in inter-lobe dynamics and the sampling of catalytically competent conformations for helix alphaC and the activation segment rather than a transition to a static active conformation underlies Eph RTK activation.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16977320}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16977320 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16977320}}
==About this Structure==
==About this Structure==
Line 28: Line 32:
[[Category: Activation]]
[[Category: Activation]]
[[Category: Eph kinase domain]]
[[Category: Eph kinase domain]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:11:55 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:51:16 2008''

Revision as of 01:51, 28 July 2008

Image:2hen.png

Template:STRUCTURE 2hen

Crystal Structure of the EphB2 Receptor Kinase domain in complex with ADP

Template:ABSTRACT PUBMED 16977320

About this Structure

2HEN is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

A change in conformational dynamics underlies the activation of Eph receptor tyrosine kinases., Wiesner S, Wybenga-Groot LE, Warner N, Lin H, Pawson T, Forman-Kay JD, Sicheri F, EMBO J. 2006 Oct 4;25(19):4686-96. Epub 2006 Sep 14. PMID:16977320

Page seeded by OCA on Mon Jul 28 04:51:16 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2hen"
Personal tools